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Recombinant Danio rerio Plexin A3 (plxna3), partial

Recombinant Danio rerio Plexin A3 (plxna3), partial

Code	CSB-YP018219DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP018219DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP018219DIL-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP018219DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP018219DIL
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

plxna3

Uniprot No.

B0S5N4

Alternative Names

plxna3; si:dkey-7i23.3; Plexin A3

Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Coreceptor for class 3 semaphorins. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.

Gene References into Functions

\Homodimerization of PlxnA3 caused by mutation M1281L remains in the presence of ligand semaphorin 3F and co-receptor neuropilin-2a. PMID: 25565389
Data show that null mutants of the guidance receptor plexin A3 display motor axon branching defects. PMID: 23349787
in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding PMID: 23302694
study shows plexinA3 is a crucial receptor for axon guidance cues in primary motor neurons PMID: 17475806
Plexin A3 plays an additional role in motor axonal morphogenesis. PMID: 17699603
Plxna3 acts with its ligand Sema3a1 for fasciculation and correct target selection of the Vp and VII motor axons. PMID: 17699608