Recombinant Danio rerio Survival motor neuron protein 1 (smn1)

Code CSB-YP895248DIL
MSDS
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Source Yeast
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Code CSB-EP895248DIL
MSDS
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Source E.coli
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Code CSB-EP895248DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895248DIL
MSDS
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Source Baculovirus
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Code CSB-MP895248DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
smn1; smn; Survival motor neuron protein 1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-281
Target Protein Sequence
MANGAEDVVF CRGTGQSDDS DIWDDTALIK AYDKAVASFK NALKGEDGAT PQENDNPGKK RKNNKKNKSR KRCNAAPDKE WQVGDSCYAF WSEDGNLYTA TITSVDQEKG TCVVFYTDYG NEEEQNLSDL LTEPPDMDED ALKTANVKET ESSTEESDRS FTPQKSGHAK HKSKSNFPMG PPSWFPSFPP GPPPPPPHFK KMDGRRGEGP GPSFPGWPPM IPLGPPMIPP PPPMSPDFGE DDEALGSMLI SWYMSGYHTG YYMGLRQGRK EAAASKKSHR K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs. Required in motor neurons and proprioceptive neurons to ensure correct U12 intron splicing and proper levels of tmem41b mRNA. Required for the maturation of motor neuron axonal branches and dendrites.
Gene References into Functions
  1. we established that the additional supply of Smn protein in motoneurons is necessary for proper axonogenesis in a cell-autonomous manner PMID: 27273160
  2. In motor neurons, smn1 interacts with the RNA binding protein, HuD and regulates motoneuron development and function. PMID: 29061699
  3. our finding that elevation of Pgk1 levels or activity, via injection of Pgk1 mRNA or treatment with terazosin respectively, robustly ameliorated MN pathology in smn morphant zebrafish provides an initial demonstration that these pathways are amenable to therapeutic intervention. PMID: 28426667
  4. Reduction of Gemin2, unlike reduction of SMN, in zebrafish embryos does not directly cause motor axon outgrowth defects. PMID: 18000835
  5. SMN deficiency affects splicing and abundance of nrxn2a. This may explain the pre-synaptic defects at neuromuscular endplates in SMA pathophysiology. PMID: 24218366
  6. SMN is needed early in development of motoneuron dendrites and axons to develop normally and that this is essential for proper connectivity and movement. PMID: 23459934
  7. These results show that survival motor neuron functions in motor axon development and suggest that these early developmental defects may lead to subsequent motoneuron loss. PMID: 12952942
  8. Survival motor neuron has a novel function in motor neurons independent of small nuclear ribonucleoprotein (snRNP) biosynthesis. PMID: 17065443
  9. Since Gemin2 and SMN both function in snRNP biogenesis yet only SMN knockdown causes motor axon defects, these data are consistent with an additional role for SMN that is snRNP independent. PMID: 18000835
  10. Smn mutants in zebrafish survive to larval stages and exhibit presynaptic neuromuscular junction defects. PMID: 19592581

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Subcellular Location
Nucleus, gem. Nucleus, Cajal body. Cytoplasm. Cytoplasmic granule. Perikaryon. Cell projection, neuron projection. Cytoplasm, myofibril, sarcomere, Z line.
Protein Families
SMN family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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