Recombinant Danio rerio Transcription factor Sox-3 (sox3)

Code CSB-YP738605DIL
MSDS
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Source Yeast
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Code CSB-EP738605DIL
MSDS
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Source E.coli
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Code CSB-EP738605DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP738605DIL
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP738605DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sox3
Uniprot No.
Alternative Names
sox3; zgc:110279; Transcription factor Sox-3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-300
Target Protein Sequence
MYNMMETEIK SPIPQSNTGS VTGGKNNSAN DQDRVKRPMN AFMVWSRGQR RKMAQENPKM HNSEISKRLG ADWKLLTDAE KRPFIDEAKR LRAMHMKEHP DYKYRPRRKT KTLLKKDKYS LPGGLLAPGA NAVNNAVSVG QRMDYTHMNG WTNSAYSLMQ DQLAYPQHPS MNSPQIQQMH RYDMAGLQYP MMSTAQTYMN AASTYSSMSP AYTQQTSSAM GLGSMASVCK TEPSSPPPAI TSHSQRACLG DLRDMISMYL PPGGDSADHS SLQTSRLHSV HPHYQSAGTG VNGTLPLTHI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator.
Gene References into Functions
  1. these findings show that sox2 and sox3 are together required for proper otic induction, but the level of expression must be tightly regulated to avoid suppression of differentiation and maintenance of pluripotency. PMID: 29355522
  2. these data show that sox2 and sox3 exhibit intrinsic differences in promoting sensory vs. neural competence, but at high levels these factors can mimic each other to enhance both states. Regional cofactors like pax2a and fgf8 also modify sox2/3 functions. PMID: 29355523
  3. significant decreases in sox3 (up to 3-fold) expression following chilling. Significant increases in expression of sox3 (up to 33-fold) during warming of chilled zebrafish embyos. PMID: 21820425
  4. Knockdown of fgf24 or sox3 causes severe epibranchial deficiencies but has little effect on otic development PMID: 21215261
  5. Knockdown of the four B1 sox genes sox2/3/19a/19b resulted in severe developmental abnormalities. PMID: 20463883
  6. The pro-neural effects of Sox3 can compensate for inhibition of fibroblast growth factor (Fgf) signalling in inducing neural tissue but it is not sufficient to maintain neural fate, suggesting the presence of Sox3-independent roles of Fgf at later stages. PMID: 18572157

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Subcellular Location
Nucleus.
Database Links
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