Recombinant Danio rerio Tyrosyl-DNA phosphodiesterase 2 (tdp2)

Code CSB-YP723059DIL
MSDS
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Source Yeast
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Code CSB-EP723059DIL
MSDS
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Source E.coli
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Code CSB-EP723059DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723059DIL
MSDS
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Source Baculovirus
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Code CSB-MP723059DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tdp2
Uniprot No.
Alternative Names
tdp2; ttrap; ttrapl; si:ch211-81e5.5; si:dkey-218n20.5Tyrosyl-DNA phosphodiesterase 2; Tyr-DNA phosphodiesterase 2; EC 3.1.4.-; 5'-tyrosyl-DNA phosphodiesterase; 5'-Tyr-DNA phosphodiesterase; TRAF and TNF receptor-associated protein homolog
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-369
Target Protein Sequence
MSALGESRTR LCDQFAFVSG SDSAVAQCYL AENEWDMERA LNSFFEAHMD SVFDEEAEKT EVTGNKRKDD TAEASGTKKK LKTDNADCID LTAEEPTCSI TVNSKENQAE NGTAKSEVED SKLSIISWNV DGLDTLNLAD RARGLCSYLA LYTPDVVFLQ ELIPAYVQYL KKRAVSYLFF EGSDDGYFTG IMLRKSRVKF LESEIICFPT TQMMRNLLIA QVTFSGQKLY LMTSHLESCK NQSQERTKQL RVVLQKIKEA PEDAIVIFAG DTNLRDAEVA NVGGLPAGVC DVWEQLGKQE HCRYTWDTKA NSNKTVPYVS RCRFDRIFLR SAKTAPPVTP DHMALIGMEK LDCGRYTSDH WGIYCTFNT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (top2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. Controls gastrulation movements and left/right (L/R) axis determination via smad3-mediated regulation of cdh1/e-cadherin. Regulates the formation of Kupffer's vesicle, a signaling center essential for establishing L/R asymmetry. Modulates smad3 activity through modulating nodal-acvr1/akt4 signaling.
Subcellular Location
Nucleus. Nucleus, PML body.
Protein Families
CCR4/nocturin family, TTRAP/TDP2 subfamily
Tissue Specificity
Expressed ubiquitously during blastula stages and throughout gastrulation. Shortly after shield formation, expressed weakly in dorsal forerunner cells (DFCs). Between somite stages 5 and 9, expressed in the tailbud and around the Kupffer's vesicle at a hi
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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