Recombinant Dog Agouti-signaling protein (ASIP)

Code CSB-YP736035DO
MSDS
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Source Yeast
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Code CSB-EP736035DO
MSDS
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Source E.coli
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Code CSB-EP736035DO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP736035DO
MSDS
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Source Baculovirus
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Code CSB-MP736035DO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ASIP
Uniprot No.
Alternative Names
ASIP; Agouti-signaling protein; ASP; Agouti switch protein
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
23-131
Target Protein Sequence
HLAEEKPK DDRSLRSNSS VNLLDFPSVS IVALNKKSKK ISRKEAEKKR SSKKKASMKN VARPRPPPPT PCVATRNSCK SPAPACCDPC ASCQCRFFRS ACTCRVLSPR C
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment).
Gene References into Functions
  1. The results of this study suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs. PMID: 22524303
  2. A SINE insertion in intron 1 of ASIP causes the black-and-tan and saddle tan phenotypes in domestic dogs. PMID: 21846741
  3. 2 key mutations in the MC1R gene and the CBD103 gene are associated with the phenotypic discriminations among brindle, red/sesame, and white coats. PMID: 21321476
  4. Mc1r is epistatic to variation at Agouti or K and the epistatic relationship between Agouti and K depends on the alleles being tested. PMID: 17483404
  5. Coyote-dog hybrids were heterozygous for 2 mutations causing fawn coat color in dogs. PMID: 17630272

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Subcellular Location
Secreted.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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