Recombinant Drosophila melanogaster Achaete-scute complex protein T5 (ac)

Code CSB-YP318709DLU
MSDS
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Source Yeast
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Code CSB-EP318709DLU
MSDS
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Source E.coli
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Code CSB-EP318709DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318709DLU
MSDS
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Source Baculovirus
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Code CSB-MP318709DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ac
Uniprot No.
Alternative Names
ac; T5; CG3796Achaete-scute complex protein T5; Protein achaete
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-201
Target Protein Sequence
MALGSENHSV FNDDEESSSA FNGPSVIRRN ARERNRVKQV NNGFSQLRQH IPAAVIADLS NGRRGIGPGA NKKLSKVSTL KMAVEYIRRL QKVLHENDQQ KQKQLHLQQQ HLHFQQQQQH QHLYAWHQEL QLQSPTGSTS SCNSISSYCK PATSTIPGAT PPNNFHTKLE ASFEDYRNNS CSSGTEDEDI LDYISLWQDD L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
Gene References into Functions
  1. Proneural proteins Achaete and Scute associate with nuclear actin to promote formation of external sensory organs. PMID: 24190881
  2. Achate-scute is transcriptionally regulated by Suppressor of Hairy wing and Modifier of MDG4 PMID: 12783795
  3. overexpression of chn enhances expression and the formation of extra sensory organs PMID: 15703278
  4. achaete, but not scute, is dispensable for development of the peripheral nervous system of Drosophila. PMID: 16084506
  5. The general model for generation of periodic pattern in the adult leg is a process involving broad and late activation of achaete PMID: 16542648
  6. quantified the relationship between achaete-scute complex (ASC) polymorphisms and bristle number phenotypes in several new population samples PMID: 17277365
  7. Dichaete can physically interact with Ind and Vnd in the Drosophila central nervous system PMID: 17360441
  8. Studies of the AS-C has contributed to the understanding of eukaryotic gene organization and the dissection of the developmental mechanisms underlying pattern formation. PMID: 19622761

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Tissue Specificity
L(1)SC, SC and AC strongly label the presumptive stomatogastric nervous system, while ASE is more prominent in the presumptive procephalic lobe.
Database Links

KEGG: dme:Dmel_CG3796

STRING: 7227.FBpp0070071

UniGene: Dm.25370

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