Recombinant Drosophila melanogaster Actin-related protein 2 (Arp14D)

Code CSB-YP001248DLU
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Source Yeast
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Code CSB-EP001248DLU
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Source E.coli
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Code CSB-EP001248DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001248DLU
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Source Baculovirus
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Code CSB-MP001248DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arp2
Uniprot No.
Alternative Names
Arp2; Actr14D; Arp14D; CG9901Actin-related protein 2; Actin-like protein 14D; Actin-like protein 2
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-394
Target Protein Sequence
MDSKGRNVIV CDNGTGFVKC GYAGSNFPTH IFPSMVGRPM IRAVNKIGDI EVKDLMVGDE ASQLRSLLEV SYPMENGVVR NWDDMCHVWD YTFGPKKMDI DPTNTKILLT EPPMNPTKNR EKMIEVMFEK YGFDSAYIAI QAVLTLYAQG LISGVVIDSG DGVTHICPVY EEFALPHLTR RLDIAGRDIT RYLIKLLLLR GYAFNHSADF ETVRIMKEKL CYIGYDIEME QRLALETTVL VESYTLPDGR VIKVGGERFE APEALFQPHL INVEGPGIAE LAFNTIQAAD IDIRPELYKH IVLSGGSTMY PGLPSRLERE IKQLYLERVL KNDTEKLAKF KIRIEDPPRR KDMVFIGGAV LAEVTKDRDG FWMSKQEYQE QGLKVLQKLQ KISH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Gene References into Functions
  1. Cofilin promotes phosphate dissociation and severs filaments, generating new pointed ends and rendering Arp2/3-generated networks competent to bind tropomyosin PMID: 26028436
  2. Arp2/3 has an essential role in the process of secretory cargo expulsion and integration of vesicular membranes with the apical plasma membrane. PMID: 26639106
  3. Abp1 and Arp2/3 complex-mediated actin cytoskeletal dynamics drive both synaptic bouton formation and neuromuscular junction branching. PMID: 24841972
  4. Arp2/3 has a role in PI(4,5)P2 regulation of myoblast fusion in Drosophila PMID: 24821989
  5. The Arp2/3 complex is required for actin cap expansion and pseudocleavage furrow formation during the syncytial blastoderm divisions. PMID: 12007413
  6. two actin regulators, capping protein, a barbed end binding protein, and the Arp2/3 complex, a potent actin assembly nucleator, function to generate properly organized bundles. PMID: 16822838
  7. The data indicate that WASp-dependent Arp2/3 actin polymerization is crucial for apical presentation of Delta, providing a mechanistic link between actin polymerization and Notch signalling. PMID: 19543274

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin family, ARP2 subfamily
Database Links

KEGG: dme:Dmel_CG9901

STRING: 7227.FBpp0302793

UniGene: Dm.183

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