Recombinant Drosophila melanogaster Amnesiac neuropeptides (amn)

Code CSB-YP638753DLU
MSDS
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Source Yeast
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Code CSB-EP638753DLU
MSDS
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Source E.coli
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Code CSB-EP638753DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP638753DLU
MSDS
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Source Baculovirus
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Code CSB-MP638753DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
amn
Uniprot No.
Alternative Names
amn; CG11937Amnesiac neuropeptides [Cleaved into: Amnesiac peptide 24; Amnesiac peptide 30; Amnesiac peptide 56]
Species
Drosophila melanogaster (Fruit fly)
Expression Region
36-59
Target Protein Sequence
RVVSG SKGSAALALC RQFEQLSAS
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for associative learning and memory in adults. Expression pattern suggests a modulatory role in memory formation. Controls neurotransmitter-mediated signaling pathways associated with the structure of the larval peripheral nerve.
Gene References into Functions
  1. These results provide compelling evidence that amn is a novel genetic component of the mechanism that regulates thermal nociception in Drosophila. PMID: 19995327
  2. brain functions from knockouts and mutants (REVIEW) PMID: 12270109
  3. Age-related memory impairment in flies results from a specific decrease in amn-dependent middle-term memory. PMID: 14659098
  4. provides support for functional homology between human PACAP-38 and the amn gene product in Drosophilas PMID: 15201281
  5. amn is required in initiation and maintenance of sleep. PMID: 18514063

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Subcellular Location
Secreted.
Tissue Specificity
Enriched expression in the embryonic and larval nervous systems. Strongly expressed in two large neurons that project over all the lobes of the mushroom bodies.
Database Links

KEGG: dme:Dmel_CG11937

STRING: 7227.FBpp0074571

UniGene: Dm.2893

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