Recombinant Drosophila melanogaster Apolipophorins (apolpp), partial

Code CSB-YP893707DLU
MSDS
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Source Yeast
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Code CSB-EP893707DLU
MSDS
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Source E.coli
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Code CSB-EP893707DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893707DLU
MSDS
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Source Baculovirus
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Code CSB-MP893707DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rfabg
Uniprot No.
Alternative Names
apolpp; Rfabg; RfaBp; CG11064Apolipophorins; Retinoid- and fatty acid-binding glycoprotein) [Cleaved into: Apolipophorin-2; ApoL2; Apolipophorin II; ApoLII); Apolipophorin-1; ApoL1; Apolipophorin I; ApoLI)]
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Constitutes the major component of lipophorin, which mediates transport for various types of lipids in hemolymph. Acts by forming lipoprotein particles that bind lipoproteins and lipids. Also involved in the transport of hydrophobic ligands like juvenile hormones, pheromone hydrocarbons and carotenoids. Required for morphogens wingless (wg) and hedgehog (hh) function, probably by acting as vehicles for the movement of wg and hh, explaining how covalently lipidated wg and hh can spread over long distances. May also be involved in transport and/or metabolism of heme.
Gene References into Functions
  1. Wingless, Hedgehog and glycophosphatidylinositol-linked proteins copurify with lipophorin, and co-localize with it in the developing wing epithelium of Drosophila. PMID: 15875013
Subcellular Location
Secreted. Note=Secreted in hemolymph.
Tissue Specificity
During stage 12, it is highly present throughout the yolk sac. By late stage 14, it localizes in the lateral fat body cells. Starting at stage 14, it localizes to the apodemes. Component of hemolymph clots (at protein level). Expressed in the amniosera.
Database Links

KEGG: dme:Dmel_CG11064

STRING: 7227.FBpp0088252

UniGene: Dm.1647

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