Recombinant Drosophila melanogaster Bloom syndrome protein homolog (mus309), partial

Code CSB-YP896091DLU
MSDS
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Source Yeast
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Code CSB-EP896091DLU
MSDS
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Source E.coli
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Code CSB-EP896091DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896091DLU
MSDS
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Source Baculovirus
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Code CSB-MP896091DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Blm
Uniprot No.
Alternative Names
Blm; mus309; CG6920Bloom syndrome protein homolog; Dmblm; EC 3.6.4.12; Bloom syndrome helicase ortholog; Mutagen-sensitive protein 309; RecQ helicase homolog
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.; ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. Participates in DNA replication and repair. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction.
Gene References into Functions
  1. Study shows that meiotic crossover patterning is lost in Drosophila melanogaster mutants that lack the Bloom syndrome helicase. PMID: 27989672
  2. Blm helicase function plays a role in homologous recombination. Blm is not required for suppression of recombination between diverged sequences. PMID: 28912341
  3. Dme-miR-314-3p modulation in Cr(VI) exposed Drosophila affects DNA damage repair by targeting mus309 PMID: 26590872
  4. Drosophila BLM suppresses error-prone alternative end-joining repair of DNA double-strand breaks that can result in genome instability and tumor formation during aging. PMID: 22183041
  5. these studies of synthetic lethality provide insights into the multiple functions of DmBLM and how various endonucleases may function when DmBLM is absent PMID: 22022278
  6. Data show that excision screens in mus309 mutant flies that lack DmBlm increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements. PMID: 19687136
  7. Coding sequence of Ku70 gene is wild-type in two mus309 mutant lines. PMID: 12206368
  8. results suggest a model in which BLM maintains genomic stability by promoting efficient repair DNA synthesis and thereby prevents double-strand break repair by less precise pathways PMID: 12522255
  9. model in which DmBlm acts downstream of strand invasion to unwind a D-loop intermediate to free the newly synthesized strand PMID: 15501916
  10. Blm mutants are defective for homologous repair but show a compensating increase in single-strand annealing. PMID: 17075047
  11. DmBLM combines DNA strand displacement with DNA strand annealing to catalyze the displacement of one DNA strand while annealing a second complementary DNA strand. PMID: 17272294
  12. Mutations that delete the N terminus of DmBlm, but not the helicase domain, have DSB repair defects as severe as those caused by null mutations. PMID: 17507683
  13. mutations in mus81 and mms4 are synthetically lethal with null mutations in mus309 PMID: 17603121
  14. The suppression of homeologous single strand annealing depends on the Bloom helicase (Blm), encoded by the Drosophila gene mus309. PMID: 18978019

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Subcellular Location
Nucleus.
Protein Families
Helicase family, RecQ subfamily
Database Links

KEGG: dme:Dmel_CG6920

STRING: 7227.FBpp0081910

UniGene: Dm.2444

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