Recombinant Drosophila melanogaster Brain tumor protein (brat), partial

Code CSB-EP839760DLU
MSDS
Size $306
Order now
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
brat
Uniprot No.
Research Area
Others
Species
Drosophila melanogaster (Fruit fly)
Source
E.coli
Expression Region
294-487aa
Target Protein Sequence
QANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
26
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
A NHL-domain family protein that functions a translational repressor to inhibit cell proliferation. Plays a central role in translation repression of hb mRNA by being recruited by nos and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. During neuroblast division, segregates asymmetrically and inhibits self-renewal of one of the two daughter cells. Together with the asymmetrically segregating transcription factor prospero ensures that the daughter cell will stop growing, exit the cell cycle, and differentiate into neurons possibly by modulating the function of dm in ganglion mother cells (GMC). Restrics developmental potential of type II intermediary neuronal progenitor (INP) cells playing a role in proliferation and maturation of the neuroblasts.
Gene References into Functions
  1. Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor proteins has been described. PMID: 28318367
  2. Data suggest that brain tumor protein (Brat) promotes midline crossing through directing the localization or stability of Adenomatous polyposis coli-2 (Apc2) at the plus ends of microtubules in navigating commissural axons. PMID: 29617376
  3. results support a novel role for Brat/TRIM3 in maintaining stem cell equilibrium and suppressing tumor growth by regulating NICD nuclear transport PMID: 26893479
  4. BRAT is a prominent post-transcriptional regulator in the early embryo through mechanisms that are largely independent of PUM. PMID: 25962635
  5. that Brat represses the translation of src64B, an upstream regulator of a conserved Rho-dependent pathway previously shown to promote axon retraction PMID: 25297111
  6. the Brat-dependent mechanism extinguishes the function of the self-renewal factor Klu in the presumptive intermediate progenitor cell by attenuating Arm activity, balancing stem cell maintenance and progenitor cell specification. PMID: 24257623
  7. These results together reveal an important and previously unidentified role for Brat in synaptic development and endocytosis mediated by suppression of BMP signaling. PMID: 23884941
  8. Cofactors Brain Tumor and eIF4E Homologous Protein are not obligatory for Pumilio and Nanos activity. PMID: 22064486
  9. Brain Tumor (Brat) was identified as a potent differentiation factor and target of Pum-Nos regulation. PMID: 21238926
  10. We show that Drosophila larval neuroblasts-stem cell-like precursors of the adult brain-regulate proliferation by segregating the growth inhibitor Brat and the transcription factor Prospero into only one daughter cell. PMID: 16564014
  11. Results suggest that the tumour suppressor brat negatively regulates cell proliferation during larval brain development in Drosophila, and that Prospero acts as a key downstream effector of brat in cell fate specification and proliferation control. PMID: 16774999

Show More

Hide All

Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Tissue Specificity
Expressed during embryogenesis, mainly in nervous tissues. Expressed in the embryonic central and peripheral nervous systems including the embryonic brain. In third instar larva it is expressed in the larval central nervous system including the brain and
Database Links

KEGG: dme:Dmel_CG10719

STRING: 7227.FBpp0293081

UniGene: Dm.6426

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*