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brat Antibody

Datasheet
Code CSB-PA839760XA01DLU
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: brat Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 31 kDa
    Observed band size: 31 kDa
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Product Details

Uniprot No.
Q8MQJ9
Target Names
brat
Alternative Names
Brain tumor protein brat CG10719
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) brat protein (294-487aa)
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To obtain the brat polyclonal antibody, a recombinant Drosophila melanogaster (Fruit fly) brat protein segment (amino acids 294-487) is used as the immunogen to stimulate an antibody response in a rabbit. The serum collected from the rabbit contains polyclonal antibodies, which are subsequently purified using affinity chromatography. The proficiency of the brat antibody in detecting the Drosophila melanogaster (Fruit fly) brat protein is established through ELISA and WB assays, confirming its reliability for experimental applications.

In Drosophila melanogaster (fruit fly), the brat (brain tumor) protein is a crucial regulator of neuroblast differentiation during nervous system development. Functioning as a tumor suppressor, brat plays a pivotal role in orchestrating asymmetric cell division in neuroblasts, ensuring the balanced production of cells with distinct fates. By controlling cell fate determination and preventing uncontrolled proliferation, brat contributes to the formation and maintenance of a properly functioning nervous system.

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Target Background

Function
A NHL-domain family protein that functions a translational repressor to inhibit cell proliferation. Plays a central role in translation repression of hb mRNA by being recruited by nos and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. During neuroblast division, segregates asymmetrically and inhibits self-renewal of one of the two daughter cells. Together with the asymmetrically segregating transcription factor prospero ensures that the daughter cell will stop growing, exit the cell cycle, and differentiate into neurons possibly by modulating the function of dm in ganglion mother cells (GMC). Restrics developmental potential of type II intermediary neuronal progenitor (INP) cells playing a role in proliferation and maturation of the neuroblasts.
Gene References into Functions
  1. Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor proteins has been described. PMID: 28318367
  2. Data suggest that brain tumor protein (Brat) promotes midline crossing through directing the localization or stability of Adenomatous polyposis coli-2 (Apc2) at the plus ends of microtubules in navigating commissural axons. PMID: 29617376
  3. results support a novel role for Brat/TRIM3 in maintaining stem cell equilibrium and suppressing tumor growth by regulating NICD nuclear transport PMID: 26893479
  4. BRAT is a prominent post-transcriptional regulator in the early embryo through mechanisms that are largely independent of PUM. PMID: 25962635
  5. that Brat represses the translation of src64B, an upstream regulator of a conserved Rho-dependent pathway previously shown to promote axon retraction PMID: 25297111
  6. the Brat-dependent mechanism extinguishes the function of the self-renewal factor Klu in the presumptive intermediate progenitor cell by attenuating Arm activity, balancing stem cell maintenance and progenitor cell specification. PMID: 24257623
  7. These results together reveal an important and previously unidentified role for Brat in synaptic development and endocytosis mediated by suppression of BMP signaling. PMID: 23884941
  8. Cofactors Brain Tumor and eIF4E Homologous Protein are not obligatory for Pumilio and Nanos activity. PMID: 22064486
  9. Brain Tumor (Brat) was identified as a potent differentiation factor and target of Pum-Nos regulation. PMID: 21238926
  10. We show that Drosophila larval neuroblasts-stem cell-like precursors of the adult brain-regulate proliferation by segregating the growth inhibitor Brat and the transcription factor Prospero into only one daughter cell. PMID: 16564014
  11. Results suggest that the tumour suppressor brat negatively regulates cell proliferation during larval brain development in Drosophila, and that Prospero acts as a key downstream effector of brat in cell fate specification and proliferation control. PMID: 16774999

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Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Tissue Specificity
Expressed during embryogenesis, mainly in nervous tissues. Expressed in the embryonic central and peripheral nervous systems including the embryonic brain. In third instar larva it is expressed in the larval central nervous system including the brain and
Database Links

KEGG: dme:Dmel_CG10719

STRING: 7227.FBpp0293081

UniGene: Dm.6426

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