Product Details

Purity

>85% (SDS-PAGE)

Target Names

slo

Uniprot No.

Q03720

Alternative Names

slo; CG10693; Calcium-activated potassium channel slowpoke; dSlo; BK channel; Maxi K channel; MaxiK

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Kinetics are determined by alternative splicing, phosphorylation status and its combination interaction with Slob and 14-3-3-zeta. While the interaction with Slob1 alone increases its activity, its interaction with both Slob1 and 14-3-3-zeta decreases its activity.

Gene References into Functions

Removal of element 6b from the endogenous slo gene affected the production of functional ethanol tolerance in an ethanol-vapor recovery from sedation assay. PMID: 26992698
slowpoke channels are required specifically in motoneurones for maximum firing rates during locomotion. PMID: 26332699
SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels PMID: 26823461
Slo mutant larvae exhibit alterations in synapse morphology, active zone size and neurotransmission that are similar to dysc mutants. PMID: 25359729
Results show using the Drosophila model organism, we demonstrate a central role for the BK-type Ca(2+) -activated K(+) channel gene slo in the production of alcohol withdrawal seizures PMID: 22734584
Here we examined the action of docosahexaenoic acid on the Slo1 channel without any auxiliary subunit and sought to elucidate the biophysical mechanism and the molecular determinants of the DHA sensitivity. PMID: 24127525
Our work identifies a Drosophila homolog of a deaf-blindness gene as a new component of the circadian output pathway,termed DYSC(dyschronic) whish expressed in major neuronal tracts and regulates expression of SLOWPOKE PMID: 22532808
Our work identifies a Drosophila homolog of a deaf-blindness gene as a new component of the circadian output pathway,termed DYSC(dyschronic) whish expressed in major neuronal tracts and regulates expression of SLOWPOKE (SLO) PMID: 22532808
Involved with Slob protein in the circadian regulation of rhythmic locomotor behavior PMID: 12417656
the slowpoke channel must be involved in triggering or producing a homeostatic mechanism that opposes the sedative effects of ethanol. PMID: 16269907
The Drosophila slowpoke gene includes 14 alternative exons distributed among five sites of alternative splicing. Slowpoke transcripts undergo tissue- and developmental-specific splicing in Drosophila and some exons are diagnostic for specific tissues. PMID: 16716202
These results implicate distinct calcium-dependent and -independent mechanisms in the modulation of dSlo by Slob PMID: 17074977

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Potassium channel family, Calcium-activated (TC 1.A.1.3) subfamily, Slo sub-subfamily

Tissue Specificity

Expressed in muscle cells, neurons of the CNS and PNS, mushroom bodies, a limited number of cells in embryonic and larval midgut and in epithelial-derived tracheal cells. During pupariation and embryogenesis, it is expressed in muscles many hours prior to

Database Links

KEGG: dme:Dmel_CG10693

UniGene: Dm.19520

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Recombinant Drosophila melanogaster Calcium-activated potassium channel slowpoke (slo), partial

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