Recombinant Drosophila melanogaster Casein kinase II subunit beta (CkIIbeta)

Code CSB-YP006075DLU
MSDS
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Source Yeast
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Code CSB-EP006075DLU
MSDS
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Source E.coli
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Code CSB-EP006075DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006075DLU
MSDS
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Source Baculovirus
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Code CSB-MP006075DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CkIIbeta
Uniprot No.
Alternative Names
CkIIbeta; Cask-II-b; CG15224Casein kinase II subunit beta; CK II beta
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-235
Target Protein Sequence
MSSSEEVSWV TWFCGLRGNE FFCEVDEDYI QDKFNLTGLN EQVPNYRQAL DMILDLEPED ELEDNPLQSD MTEQAAEMLY GLIHARYILT NRGIAQMIEK YQTGDFGHCP RVYCESQPML PLGLSDIPGE AMVKTYCPKC IDVYTPKSSR HHHTDGAYFG TGFPHMLFMV HPEYRPKRPT NQFVPRLYGF KIHSLAYQIQ LQAAANFKMP LRAQRGQPPK DEEPENNADT VPKRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Gene References into Functions
  1. Data (including data from studies using transgenic animals) suggest that catalytic activity of casein kinase 2 (CkIIa/CkIIb) is essential for oogenesis in this species; Jabba, a lipid droplet-associated protein and substrate for casein kinase 2, is essential in modulating ovarian lipid metabolism. (CkIIa = casein kinase II alpha subunit; CkIIb = casein kinase II beta subunit) PMID: 29326167
  2. Drosophila splicing factor P element Somatic Inhibitor (PSI) is phosphorylated at at least two different sites by at minimum two different kinases, casein kinase II (CK II) and tousled-like kinase (tlk). PMID: 23437125
  3. Coexpressing CK2alpha and CK2beta increases Smo accumulation and induces ectopic Hh target gene expression. PMID: 20876583
  4. Identification and characterization of proteins that interact with Drosophila melanogaster protein kinase CK2 PMID: 11827179
  5. ZFP47 is a new physiological partner and substrate of CK2. PMID: 11827180
  6. CK2(beta)tes encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 PMID: 11874456
  7. This study implicates a role for DmCK2beta in cell proliferation or cell survival during brain development. PMID: 12406573
  8. results reveal a potential mechanism for regulation of the dMi-2 enzyme and point toward CK2 phosphorylation as a common feature of CHD family ATPases PMID: 16223721
  9. The DmCK2beta transcription unit encodes for functionally non-redundant protein isoforms. PMID: 16530986

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Protein Families
Casein kinase 2 subunit beta family
Database Links

KEGG: dme:Dmel_CG15224

STRING: 7227.FBpp0089135

UniGene: Dm.142

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