Recombinant Drosophila melanogaster Caspase-1 (Dcp-1), partial

Code CSB-YP004543DLU
MSDS
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Source Yeast
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Code CSB-EP004543DLU
MSDS
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Source E.coli
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Code CSB-EP004543DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004543DLU
MSDS
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Source Baculovirus
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Code CSB-MP004543DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dcp-1
Uniprot No.
Alternative Names
Dcp-1; CG5370Caspase-1; EC 3.4.22.-) [Cleaved into: Caspase-1 subunit p22; Caspase-1 subunit p13]
Species
Drosophila melanogaster (Fruit fly)
Expression Region
34-202
Target Protein Sequence
AKGCTPE SLVVGGATAA SPLPANKFVA RMPVERYASE YNMSHKHRGV ALIFNHEFFD IPSLKSRTGT NVDAQELKKA FENLGFAVSV HKDCKLRDIL KHVGKAAELD HTDNDCLAVA ILSHGEHGYL YAKDTQYKLD NIWHYFTATF CPSLAGKPKL FFIQACQGDR LD
Protein Length
partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Loss of zygotic DCP-1 function causes larval lethality and melanotic tumors.
Gene References into Functions
  1. drICE and dcp-1 function in cell death redundantly. However, dying neurons in a few clusters strictly required drICE but not dcp-1, but required drICE and dcp-1 when drICE activity was reduced via hypomorphic mutation. PMID: 26022392
  2. Autophagy suppresses Dcp-1-mediated apoptotic cell death, whereas Dcp-1 positively regulates autophagy, possibly through feedback regulation. PMID: 20100334
  3. novel characteristic morphological features of egg chambers lacking both dcp-1 and pita functions in the germline cells; suggested an essential role of dcp-1 and/or pita during mid-oogenesis PMID: 15547953
  4. A double-mutant analysis between drICE and death caspase-1 (dcp-1), another effector caspase, reveals that some cells (type I) strictly require drICE for apoptosis, whereas other cells (type II) require either drICE or dcp-1. PMID: 16645642
  5. Data show that the effector caspase Dcp-1 and the inhibitor of apoptosis protein Bruce function to regulate both autophagy and starvation-induced cell death in Drosophila. PMID: 18794330
  6. HeT-A mRNA is derepressed in mRNA degradation mutants dcp1, indicating that the enzyme also aid in removing full-length transcripts and/or decay intermediates. PMID: 19651888

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Protein Families
Peptidase C14A family
Database Links

KEGG: dme:Dmel_CG5370

STRING: 7227.FBpp0071971

UniGene: Dm.4849

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