Recombinant Drosophila melanogaster Chorion transcription factor Cf2 (Cf2), partial

Code CSB-YP321800DLU
MSDS
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Source Yeast
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Code CSB-EP321800DLU
MSDS
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Source E.coli
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Code CSB-EP321800DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321800DLU
MSDS
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Source Baculovirus
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Code CSB-MP321800DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cf2
Uniprot No.
Alternative Names
Cf2; CG11924; Chorion transcription factor Cf2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator; binds to the promoter region of Cp15. Also binds to its own promoter, thus having a probable autoregulatory role.
Gene References into Functions
  1. CF2 expression variations yield altered Mef2 expression levels but with correct spatio-temporal Mef2 expression patterns, it can be concluded that only the mechanism controlling expression levels is de-regulated. Here, it is proposed that CF2 regulates Mef2 expression through a Feedforward Loop circuit. PMID: 28617826
  2. These results indicate a role for CF2 as a direct negative regulator of the thin filament protein gene Actin 88F. PMID: 20520827
  3. CF2 expression during muscle development is dependent on the myogenic factor MEF2. PMID: 12204268
  4. Mef2 and Cf2 collaborate in activation of the myogenic program in Drosophila. PMID: 18160709
  5. CF2 depletion alters the relative expression of thin and thick filament components of muscle. PMID: 18448314

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Subcellular Location
Nucleus.
Tissue Specificity
Isoform I is found in embryos, pupae and adult somatic tissue; isoform II occurs in embryos, pupae, ovaries, testis and to a lesser extent in adult somatic tissue.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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