Recombinant Drosophila melanogaster Chromodomain-helicase-DNA-binding protein Mi-2 homolog (Mi-2), partial

Code CSB-YP528539DLU
MSDS
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Source Yeast
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Code CSB-EP528539DLU
MSDS
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Source E.coli
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Code CSB-EP528539DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528539DLU
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Source Baculovirus
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Code CSB-MP528539DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mi-2
Uniprot No.
Alternative Names
Mi-2; CG8103Chromodomain-helicase-DNA-binding protein Mi-2 homolog; EC 3.6.4.12; ATP-dependent helicase Mi-2; dMi-2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Helicase which acts in nucleosome-remodeling by catalyzing ATP-dependent nucleosome mobilization. Involved in regulating transcription. Plays a vital role in development. Binds to a portion of Hunchback (HB) protein that is critical for repression of bithorax complex (BXC) genes. May also function in polycomb group (PcG) repression of Hox genes. May also act as part of the nucleosome remodeling and deacetylase complex (the NuRD complex) which participates in the remodeling of chromatin by deacetylating histones.
Gene References into Functions
  1. Genetic experiments in Drosophila demonstrate that expression of cancer-derived dMi-2 mutants misregulates differentiation of epithelial wing structures and produces phenotypes that correlate with their nucleosome remodelling properties. PMID: 29844320
  2. EcR and dMi-2 form a complex that is devoid of Ultraspiracle. Unexpectedly, EcR contacts the dMi-2 ATPase domain and increases the efficiency of dMi-2-mediated nucleosome remodelling PMID: 28378812
  3. dMi-2 is an important regulator of both chromosome condensation and cohesin binding in interphase cells. PMID: 22912596
  4. The results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity. PMID: 22362736
  5. chromodomains are DNA binding modules important for ATP-dependent nucleosome mobilization PMID: 12006495
  6. Results suggest that Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation. PMID: 16961588

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Subcellular Location
Nucleus. Chromosome.
Protein Families
SNF2/RAD54 helicase family
Database Links

KEGG: dme:Dmel_CG8103

STRING: 7227.FBpp0099808

UniGene: Dm.28317

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