Recombinant Drosophila melanogaster Cyclic AMP response element-binding protein A (CrebA), partial

Code CSB-YP328927DLU
MSDS
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Source Yeast
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Code CSB-EP328927DLU
MSDS
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Source E.coli
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Code CSB-EP328927DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328927DLU
MSDS
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Source Baculovirus
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Code CSB-MP328927DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CrebA
Uniprot No.
Alternative Names
CrebA; Bbbf2; CG7450Cyclic AMP response element-binding protein A; cAMP response element-binding protein A; dCREB-A; Box B-binding factor 2; BBF-2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator. Binds to fat body-specific enhancers of alcohol dehydrogenase (ADH) and yolk protein genes. BBF-2 may play a role in fat body gene expression. It binds the consensus sequence 5'-T[AC]NACGTAN[TG]C-3'.
Gene References into Functions
  1. Data suggest that CrebA is involved in regulation of infection tolerance in this species; expression of CrebA is regulated in the fat body upon infection; CrebA is required to survive infection and promotes tolerance of bacterial infection; CrebA deficiency leads to ER (endoplasmic reticulum) stress upon bacterial challenge. PMID: 29394281
  2. CrebA deficiency leads to ER stress upon bacterial challenge PMID: 29394281
  3. Drosophila CrebA directly binds the enhancers of secretory pathway genes and is both necessary and sufficient to activate expression of every secretory pathway component gene examined thus far. PMID: 21041443
  4. direct and indirect interactions of the hsromega transcripts with a variety of regulatory proteins like hnRNPs, CBP, proteasome, Drosophila inhibitor of apoptosis protein 1 (DIAP1) PMID: 20065067
  5. AP-1 functions upstream of CREB to control synaptic plasticity in Drosophila PMID: 11976688
  6. CrebA activity is linked to secretory function in multiple tissues PMID: 15901661
  7. cAMP-response element-binding protein and heat-shock protein 70 additively suppress polyglutamine-mediated toxicity. PMID: 16009936
  8. The CREB binding protein transcriptional cofactor is necessary and sufficient for down-regulation of DIAP1. PMID: 17591924

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Subcellular Location
Nucleus.
Protein Families
BZIP family
Tissue Specificity
In all cell types examined, including developing salivary gland in embryos and in adults, brain and optic lobe cell bodies, salivary gland, midgut epithelial cells of the cardia, female ovarian columnar follicle cells and male seminal vesicle, ejaculatory
Database Links

KEGG: dme:Dmel_CG7450

STRING: 7227.FBpp0089339

UniGene: Dm.6979

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