Product Details

Purity

>85% (SDS-PAGE)

Target Names

shg

Uniprot No.

Q24298

Alternative Names

shg; E-cadherin; CG3722; DE-cadherin; Protein shotgun

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Cadherins are calcium-dependent cell adhesion proteins. In connecting cells they preferentially interact with themselves in a homophilic manner; cadherins may thus contribute to the sorting of heterogeneous cell types. During oogenesis, integral component of the guidance mechanisms that regulate the directional persistent collective migration of the border cell (BC) cluster through the nurse cells to the oocyte. Functions downstream of the two chemoattractant receptors, Pvr and Egfr, to promote BC adhesion between the leader cells of the migrating cluster and the surrounding nurse cells. This adhesion increases Rac1 signaling in the leading cells, which in turn stabilizes DE-cadherin/DE-cadherin adhesions through the formation of forward-directed protrusions which attach/detach to the surrounding nurse cells in order to pull the cluster through the egg chamber to the oocyte. Within the BC cluster, also promotes adhesion between BCs, and between BCs and polar cells which enables the lead BC to communicate direction to the other cells in the cluster, providing polarity to each individual cell and ensuring collective behavior. May function in cell intercalation in the lateral epidermis during germband extension. Contributes to the determination of body left-right asymmetry by enhancing Myo31DF activity and inhibiting Myo61F activity.

Gene References into Functions

In disc-proper epithelial cells, DE-cadherin localizes to the zonula adherens, and in puncta at the basal, and lateral sides. Fra overexpression of affects DE-Cadherin distribution and apico-basal cell shape so that there is loss of DE-Cadherin in the basal and lateral regions. PMID: 29518139
several phospho-mutations dramatically reduced the biosynthetic turnover rate of DE-Cad during apical-basal polarization. PMID: 28219947
Transmission of cytokinesis forces via E-cadherin dilution and actomyosin flows PMID: 28296858
alpha-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. PMID: 27831494
In embryos mutant for the FGF signaling pathway components Branchless and Breathless, zygotic E-cadherin is not targeted to adherens junctions, causing midgut pocket collapse. PMID: 26604222
These results indicate an active role for E-Cad in mediating cohesive and ordered migration of non-epithelial cells, and discount the notion of E-Cad as just an epithelial feature that has to be switched off to enable migration of mesenchymal cells. PMID: 26272476
the sustained E-cadherin-dependent adhesion organizes the microvilli meshwork and ensures the proper attachment of RCs to the PM, thereby counteracting the increasing membrane tension induced by exponential tissue growth. PMID: 26424451
Hox coordination of tissue morphogenesis relies on boundary fold formation through the modulation of DE-cad positioning PMID: 26206615
The membrane-proximal half of the DE-cadherin extracellular region is dispensable for many developmental events,but it is essential for efficient and robust apical constriction during ventral furrow formation. PMID: 20184661
E-cadherin cluster organization in Drosophila embryos.Dynamin-dependent endocytosis targets large clusters of E-cadherin. PMID: 24184100
E-cadherin promotes in vivo cell movement by multiple mechanisms, the combination of which orchestrates collective direction sensing. Of particular note, E-cadherin functions at the leading edge in a positive feedback loop with the small GTPase Rac as an integral part of the direction-sensing mechanism. PMID: 24855950
E-cadherin is required to maintain Drosophila prohemocyte multipotency and block differentiation. PMID: 24040319
findings show that planar polarization of flows emerges from polarized fluctuations in the levels of E-cadherin clusters that produce transient and oscillating asymmetries of coupling; these fluctuations are triggered by polarized E-cadherin endocytosis and are amplified by flow itself PMID: 23871590
loss of DE-cadherin strongly enhances the raw mutant gonad phenotype, while increasing DE-cadherin function rescues this phenotype PMID: 22575490
findings suggest that Hrp38 and its association with poly(ADP-ribose) control germline stem cell self-renewal and oocyte localization by regulating DE-cadherin translation PMID: 22453833
DE-Cad and armadillo in adherens junctions of polarizing epithelial cells had much faster biosynthetic turnover than in polarized cells. PMID: 22159415
Cno function as a novel regulator of neuron-glia interactions, forming a complex with Shg, Wrapper and Neurexin IV. PMID: 21389054
Jafrac1-mediated reduction of hydrogen peroxide is required to maintain DE-cadherin protein levels in the early embryo. PMID: 21316590
The studies demonstrate a novel and crucial role for Pak1 and E-cadherin endocytosis in determining salivary gland lumen size and shape, and also identify a mechanism for multiple lumen formation. PMID: 21068057
E-cadherin orchestrates multiple aspects of stem cell behavior PMID: 20824213
regulates orientation of asymmetric cell division in the sensory organ lineage PMID: 11818059
study demonstrates that anchorage of germline stem cells (GSCs) in their niche by DE-cadherin-mediated adhesion is important for stem cell maintenance and function. PMID: 12052957
functions in normal optic placode morphogenesis PMID: 12163402
mediates cell adhesion and is essential for maintaining somatic stem cells in the Drosophila ovary PMID: 12393817
DE-cadherin plays a pivotal role in larval brain proliferation, brain cortex morphogenesis, and axon growth PMID: 12716940
Data show that Drosophila E-cadherin is essential both for germ cell ensheathment and gonad compaction, and is upregulated in the somatic gonad at the time of gonad formation. PMID: 12900457
DE-cadherin plays a pivotal role in delamination and polarized division of neuroblasts. PMID: 15183719
Elevated transcription of DE-cadherin is sufficient to attract the oocyte to lateral and anterior positions. PMID: 15852002
Results describe the requirements for Drosophila melanogaster epithelial (DE) cadherin regulation in vivo. PMID: 16129787
regulation of cadherin-based adherens junctions by Rac is critical for salivary gland morphogenesis and that this regulation occurs through dynamin-mediated endocytosis PMID: 16412417
zinc is a crucial regulator of developmental processes and can affect DE-cadherin expression on multiple levels PMID: 16481356
DE-cadherins integrate input from at least two signaling pathways, resulting in a coordinated cell movement PMID: 16887833
While Decapentaplegic (Dpp) acts downstream of Ras to maintain vein cell identity in the pupal wing, these results indicate that Ras controls Shg localization via a Dpp-independent mechanism. PMID: 17888420
Uncoupling stability and mobility of E-cadherin complexes suggests that stable epithelia may remodel through the regulated mobility of very stable adhesive foci. PMID: 18480755
levels of E-cadherin, an adherens junction component and Rab11-compartment cargo, are dynamically regulated by trafficking during tracheal development PMID: 18641639
Overexpressed Armadillo mutants exhibit different levels of activity and stability, but retain E-Cadherin binding. PMID: 18682750
Demonstrate that E-cadherin (E-cad) is required for stable attachment between the an intestinal stem cell and a progenitor cell. PMID: 19021776

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.

Tissue Specificity

In early stage 9 and stage 10 oocytes, expressed in border cells, strongly expressed in polar cells and very weakly expressed in the nurse cells (at protein level). In the embryo, expressed in the leading edge cells of the dorsal epidermis (at protein lev

Database Links

KEGG: dme:Dmel_CG3722

STRING: 7227.FBpp0071475

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP637588DLU
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP637588DLU
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP637588DLU-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP637588DLU
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP637588DLU
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Drosophila melanogaster DE-cadherin (shg), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage