Recombinant Drosophila melanogaster DNA-binding protein D-ETS-6 (Ets21C)

Code CSB-YP326915DLU
MSDS
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Source Yeast
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Code CSB-EP326915DLU
MSDS
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Source E.coli
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Code CSB-EP326915DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326915DLU
MSDS
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Source Baculovirus
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Code CSB-MP326915DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ets21C
Uniprot No.
Alternative Names
Ets21C; ETS-6; CG2914DNA-binding protein D-ETS-6
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-475
Target Protein Sequence
MAILQNSRQS HKQLPIISQT IRSAWCQQRP INAMHQDVRQ KSIGSGNETK LEAKETEVPT NRRRRRRRCS SSSTSDSSAS SYSSTDSDSG SSTSSSSIRS QLPALNLPVP LPLATPTPPA VSSPHQAPSP RRNSSDSNRS VSPVEVPVDP HAWTPEDIAS WVRWATRKFK LDPEPDIDRF PKDAQELCDL SRADFWVCAG SRRGGMLLAQ HFAISLYHAT GRETSPMLND DEPNPYQLLN AASHRLVAQG SGGQIQLWQF LLELLADSSN ANAISWEGQS GEFRLIDPDE VARRWGERKA KPNMNYDKLS RALRYYYDKN IMTKVHGKRY AYKFDFHGLM AACQAQAQGG DPASSMLGSY NHHAGGAMQL GRHPPPLHHH PQHSHPHHQL GQPHFLHPHH SSPASNSSSL GFPSSSTASS QASPGQAPAS SSASTSNFTA PFQGGTAGVD PARTSTSSAG NYDQGPVTPT TNAFN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Data show that Ets21C has a crucial role in the regulation of tumor growth as a loss of function critically reduced tumor growth, whereas an excess of Ets21C further increased tumor size. While Ets21C was shown only with a role in fine-tuning the transcriptional program of neoplastic tumors, results point towards a more fundamental role as activator of a specific set of target genes driving tumor growth and invasion. PMID: 27713480
  2. malignant transformation of the ras(V12)scrib(1) tumors requires bZIP protein Fos, the ETS-domain factor Ets21c and the nuclear receptor Ftz-F1, all acting downstream of Jun-N-terminal kinase. PMID: 26398940
  3. These studies identify Cic, Pnt, and Ets21C as critical downstream effectors of EGFR signaling in Drosophila Intestinal Stem Cells PMID: 26683696
Subcellular Location
Nucleus.
Protein Families
ETS family
Tissue Specificity
Embryonic ventral nervous system and 1 pair of neurons in each thoracic segment.
Database Links

KEGG: dme:Dmel_CG2914

STRING: 7227.FBpp0077739

UniGene: Dm.21518

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