Recombinant Drosophila melanogaster Deoxynucleoside kinase (dnk)

Code CSB-YP897509DLU
MSDS
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Source Yeast
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Code CSB-EP897509DLU
MSDS
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Source E.coli
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Code CSB-EP897509DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897509DLU
MSDS
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Source Baculovirus
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Code CSB-MP897509DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dnk
Uniprot No.
Alternative Names
dnk; CG5452Deoxynucleoside kinase; EC 2.7.1.145; Deoxyribonucleoside kinase; Dm-dNK; Multispecific deoxynucleoside kinase
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-250
Target Protein Sequence
MAEAASCARK GTKYAEGTQP FTVLIEGNIG SGKTTYLNHF EKYKNDICLL TEPVEKWRNV NGVNLLELMY KDPKKWAMPF QSYVTLTMLQ SHTAPTNKKL KIMERSIFSA RYCFVENMRR NGSLEQGMYN TLEEWYKFIE ESIHVQADLI IYLRTSPEVA YERIRQRARS EESCVPLKYL QELHELHEDW LIHQRRPQSC KVLVLDADLN LENIGTEYQR SESSIFDAIS SNQQPSPVLV SPSKRQRVAR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, 2'-deoxyriboadenosine, 2'-deoxyribocytidine and 2'-deoxyriboguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.
Gene References into Functions
  1. We showed that sensitivity of the osteosarcoma cell line to the nucleoside analogs and the efficiency of bystander cell killing were independent of the subcellular localization of Dm-dNK. PMID: 26134602
  2. Report synergistic antitumor effect of adenovirus armed with Drosophila melanogaster deoxyribonucleoside kinase and nucleoside analogs for human breast carcinoma. PMID: 26203222
  3. Data indicate that long term Dm-dNK ( nucleoside kinase from Drosophila melanogaster) expression rescued thymidine kinase 2 (Tk2)-deficient mice without lethal side effects. PMID: 25296759
  4. Multisubstrate deoxyribonucleoside kinase could be used in a novel therapeutic strategy for treating breast cancer and improving anti-tumor efficacy, as well as for optimizing approaches for suicide gene therapy. PMID: 21674733
  5. mechanism of feedback inhibition is presented based on the crystal structure of a complex between deoxyribonucleoside kinase and its feedback inhibitor deoxythymidine triphosphate PMID: 12741827
  6. Results describe active-site mutants of Drosophila deoxyribonucleoside kinase (Dm-DNK) with reduced phosphorylation efficiency, supporting their importance for substrate binding and/or catalysis as proposed by the recently solved structure of Dm-dNK. PMID: 12823558
  7. mutation N64D is responsible for decreasing activity towards the natural substrates PMID: 16008571
  8. This strongly suggests that dNK plays a role in cell proliferation in physiological conditions. PMID: 16582629
  9. observations give insight into the mechanisms of substrate binding and catalysis, which is important for developing novel suicide genes and drugs for use in gene therapy PMID: 17302737
  10. Structural studies of nucleoside analog and feedback inhibition binding to Drosophila melanogaster multisubstrate dnk are reported. PMID: 18384378

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Protein Families
DCK/DGK family
Database Links

KEGG: dme:Dmel_CG5452

STRING: 7227.FBpp0302815

UniGene: Dm.1248

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