Recombinant Drosophila melanogaster DnaJ protein homolog 1 (DnaJ-1)

Code CSB-YP634500DLU
MSDS
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Source Yeast
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Code CSB-EP634500DLU
MSDS
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Source E.coli
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Code CSB-EP634500DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP634500DLU
MSDS
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Source Baculovirus
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Code CSB-MP634500DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DnaJ-1
Uniprot No.
Alternative Names
DnaJ-1; DROJ1; CG10578DnaJ protein homolog 1; DROJ1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-334
Target Protein Sequence
MGKDFYKILG LERKASDDEI KKAYRKLALK YHPDKNKSPQ AEERFKEIAE AYEVLSDKKK RDIFDNYGED GLKGGQPGPD GGGQPGAYTY QFHGDPRATF AQFFGSSDPF GAFFTGGDNM FSGGQGGNTN EIFWNIGGDD MFAFNAQAPS RKRQQDPPIE HDLFVSLEEV DKGCIKKMKI SRMATGSNGP YKEEKVLRIT VKPGWKAGTK ITFPQEGDSA PNKTPADIVF IIRDKPHSLF KREGIDLKYT AQISLKQALC GALVSVPTLQ GSRIQVNPNH EIIKPTTTRR INGLGLPVPK EPSRRGDLIV SFDIKFPDTL APSLQNQLSE LLPN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Additional studies established that the increased toxicity upon mutating UbS2 stems from disrupting the autoprotective role that pathogenic ataxin-3 has against itself, which depends on the co-chaperone, DnaJ-1. Our data reveal a previously unrecognized balance between pathogenic and potentially therapeutic properties of the ataxin-3-Rad23 interaction; they highlight this interaction as critical for the toxicity of the S PMID: 28158474
  2. Through DnaJ-1, our data connect ataxin-3 and Rad23 to protective processes involved with protein folding rather than increased turnover of toxic polyQ species. PMID: 26007638
  3. Expression of DnaJ-1 potently suppresses alpha1ACT-dependent degeneration , concomitant with decreased aggregation of the pathogenic protein. Mutating the nuclear importer karyopherin a3 also leads to reduced toxicity from pathogenic CACNA1A PMID: 25954029
  4. HSP40 and HSP110 function together in protein homeostasis control. PMID: 24091676
  5. A high positive correlation was found between the levels of Hsp40 expression and scores of basal and induced thermotolerance, implying a significant contribution of Hsp40 gene in thermoadaptation. PMID: 21505045

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Subcellular Location
Cytoplasm.
Database Links

KEGG: dme:Dmel_CG10578

STRING: 7227.FBpp0076829

UniGene: Dm.2839

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