Recombinant Drosophila melanogaster Dynamin (shi), partial

Code CSB-YP326735DLU
MSDS
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Source Yeast
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Code CSB-EP326735DLU
MSDS
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Source E.coli
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Code CSB-EP326735DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326735DLU
MSDS
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Source Baculovirus
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Code CSB-MP326735DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
shi
Uniprot No.
Alternative Names
shi; CG18102Dynamin; EC 3.6.5.5; Protein shibire; dDyn
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting.
Gene References into Functions
  1. results implicate mutant dynamin as an effective seizure suppressor, suggesting that targeting or limiting the availability of synaptic vesicles could be an effective and general method of controlling epilepsy disorders PMID: 26341658
  2. These studies identify novel morphogenetic roles (i.e., tube closure and biased apical expansion), and expand upon established roles (i.e., cell intercalation and adhesion remodeling), for Dynamin in tubulogenesis. PMID: 26542010
  3. Dynamin is critically involved in the stabilization of Clathrin- and AP2-dependent endocytic pits. PMID: 24662566
  4. The shibire gene product, Dynamin, appears to be required for an immediate early response to ethanol that triggers a cellular response leading to rapid functional tolerance. PMID: 21797886
  5. A temperature sensitive shibire mutant was used to trap synaptic vesicles at the plasma membrane and extracted sterols from synaptic vesicles. PMID: 21106824
  6. Mutation in shibire causes male-male courtship behavior due to disruption of synaptic transmission PMID: 12239352
  7. The shi(ts1) mutation induces a conformational change, involving the GTP binding site, that affects dynamin aggregation. PMID: 12382260
  8. Expression of a temperature-sensitive shibire (shi) transgene in a subset of alpha/beta neurons resulted in odor-pair-specific disruption of learning. PMID: 16980542
  9. DRP mutants affect synaptic transmission in a manner similar to the Drosophila sesBorg mutants. Mitochondria with abnormal morphology in DRP mutants might lead to a deficit in ATP levels or lowered rates of ATP repletion at the synapse. PMID: 17233640

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Database Links

KEGG: dme:Dmel_CG18102

STRING: 7227.FBpp0305866

UniGene: Dm.3444

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