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Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase highwire (hiw), partial

Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase highwire (hiw), partial

Code	CSB-YP885654DLU
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP885654DLU
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP885654DLU-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP885654DLU
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP885654DLU
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

hiw

Uniprot No.

Q9NB71

Alternative Names

hiw; CG32592; E3 ubiquitin-protein ligase highwire; EC 2.3.2.-; Protein pam/highwire/rpm-1

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues. Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Required in the presynaptic motoneuron to down-regulate the levels of wnd and restrain synaptic terminal growth at the neuromuscular junction (NMJ) together with Rae1 and Fsn.

Gene References into Functions

Results indicate that the ability of faf, hiw or Rdl to modulate sleep and protect against cognitive impairments during waking is circuit dependent. Decreasing hiw and Rdl within the Mushroom Bodies protects against the negative effects of sleep deprivation as indicated by the absence of a subsequent homeostatic response, or deficits in short-term memory. PMID: 29109678
Highwire promotes axon termination, pruning and synaptogenesis. PMID: 28100586
The dual leucine zipper kinase (DLK) signaling pathway in Drosophila, which consists of Highwire and Wallenda and controls axonal growth, regeneration, and degeneration, is also involved in dendritic growth in vivo. PMID: 23750116
An increased level of Nmnat protein in hiw mutants is both required and sufficient to inhibit axonal degeneration. PMID: 23226106
Genetic interaction studies suggest that Highwire and Plexin A signals may interact to regulate normal morphogenesis of alpha/beta axons. PMID: 22131429
The regulation of Wnd protein turnover by Hiw can function as a damage surveillance mechanism for responding to axonal injury. PMID: 20921142
BMP signaling mutants suppress the excessive synaptic growth in Highwire mutants. Activation of BMP signaling in Highwire mutants causes further synaptic expansion. PMID: 15046722
Highwire may independently regulate the molecular pathways controlling synaptic growth and function. PMID: 16237161
These results suggest that Highwire controls structural plasticity of the synapse by regulating gene expression through a MAP kinase signaling pathway. PMID: 16815332

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Subcellular Location

Cell junction, synapse. Cell projection, axon.

Protein Families

Highwire family

Tissue Specificity

Express throughout the nervous system. Stage 13 embryos show expression in the central nervous system (CNS) at the longitudinal axon tracts around which the synaptic neuropil forms. Expression outside the CNS starts at stage 16 in presynaptic terminals at

Database Links

KEGG: dme:Dmel_CG32592

STRING: 7227.FBpp0073787

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