Recombinant Drosophila melanogaster ETS-like protein pointed, isoform P2/D (pnt), partial

Code CSB-YP344687DLU
MSDS
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Source Yeast
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Code CSB-EP344687DLU
MSDS
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Source E.coli
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Code CSB-EP344687DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344687DLU
MSDS
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Source Baculovirus
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Code CSB-MP344687DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pnt
Uniprot No.
Alternative Names
pnt; DMPOINT1A; ETS2; Ets58AB; pointed; CG17077; ETS-like protein pointed
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ETS transcription factor with a prominent role during development of the eye and the nervous system. Required for glial-neuronal cell interactions at the ventral midline which are necessary for the proper elaboration of commissures in the embryonic CNS.; Required for normal EGFR-induced photoreceptor development probably as a downstream effector of Ras85D. In larval eye imaginal disks, activated by MAPK phosphorylation following EGFR activation, induces transcription of isoform P1 which in turn activates transcription of target genes essential for photoreceptor development.; Required for normal EGFR-induced photoreceptor development. Following transcriptional activation by isoform P2, acts as a constitutive activator of transcription, leading to induction of target genes essential for photoreceptor development. In larval brains, involved in the maintenance of type II neuroblast self-renewal and identity together with brat, btd and pros; prevents intermediate neuronal progenitor (INP) dedifferentiation by regulating the expression of erm probably via Notch signaling; suppresses Ase expression in type II neuroblasts and promotes the generation of intermediate neuronal progenitors.
Gene References into Functions
  1. results demonstrate that PntP1 prevents both the premature differentiation and the dedifferentiation of INPs by regulating the expression of distinct target genes at different stages of imINP development. PMID: 27510969
  2. Both PntP1 and PntP2 are required for oenocyte specification. Since PntP1 is a downstream effector of EGF signaling, these findings provide insight into how a Hox factor can both trigger and potentiate the EGF signal to promote an essential cell fate along the body plan. PMID: 28715417
  3. PntP2 transcription factor plays a role in air sac development by regulation of the EGF ligand Vein protein. PMID: 26632449
  4. Specific disruption of either pntP1 or pntP2 resulted in the same R8-only phenotype, demonstrating that both Pnt isoforms are essential for photoreceptor recruitment PMID: 23757412
  5. Demonstrate that the Pointed-P2 PNT domain contains a dynamic N-terminal helix H0 appended to a core conserved five-helix bundle diagnostic of the SAM domain fold. PMID: 22936607
  6. The MAPK/ERK pathway acts via the ETS-1 transcription factor Pointed. This mechanism permits physiological adjustment of insulin sensitivity and subsequent maintenance of circulating glucose at appropriate levels. PMID: 22242005
  7. Pointed directly regulates the transcription of string and thereby cell proliferation PMID: 12447387
  8. In different types of glial cells, REPO can act alone, or cooperate with either TTK69 or PNTP1 to regulate different target genes. PMID: 12702656
  9. Data show that pannier acts upstream of pointed P2 in a developmental pathway in which pannier promotes cardiac mesoderm formation, and pointed acts subsequently to distinguish between cardioblast and pericardial cell fates. PMID: 12756183

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Subcellular Location
Nucleus.
Protein Families
ETS family
Database Links

KEGG: dme:Dmel_CG17077

STRING: 7227.FBpp0088658

UniGene: Dm.2370

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