Product Details

Purity

>85% (SDS-PAGE)

Target Names

eIF2gamma

Uniprot No.

Q24208

Alternative Names

eIF2gamma; eIF-2gamma; eIF2g; CG6476Eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit gamma; eIF-2-gamma

Species

Drosophila melanogaster (Fruit fly)

Expression Region

1-475

Target Protein Sequence

MATAEAQIGV NRNLQKQDLS NLDVSKLTPL SPEVISRQAT INIGTIGHVA HGKSTVVKAI SGVQTVRFKN ELERNITIKL GYANAKIYKC DNPKCPRPAS FVSDASSKDD SLPCTRLNCS GNFRLVRHVS FVDCPGHDIL MATMLNGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKQILILQNK IDLIKESQAK EQYEEITKFV QGTVAEGAPI IPISAQLKYN IDVLCEYIVN KIPVPPRDFN APPRLIVIRS FDVNKPGCEV ADLKGGVAGG SILSGVLKVG QEIEVRPGVV TKDSDGNITC RPIFSRIVSL FAEQNELQYA VPGGLIGVGT KIDPTLCRAD RLVGQVLGAV GQLPDIYQEL EISYYLLRRL LGVRTDGDKK GARVEKLQKN EILLVNIGSL STGGRISATK GDLAKIVLTT PVCTEKGEKI ALSRRVENHW RLIGWGQIFG GKTITPVLDS QVAKK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B.

Gene References into Functions

the SUUR protein is required for the SU(VAR)3-9 enzyme to bind to the salivary gland polytene chromosomes PMID: 21340745
These data indicate a central role for the SU(VAR)3-9 HMTase in heterochromatin-induced gene silencing in Drosophila.SU(VAR)3-9 is the major heterochromatin-specific HMTase in Drosophila. PMID: 11867540
role in chromatin organization at the histone gene cluster and in suppression of position-effect variegation PMID: 12524347
Developmental and chromosomal defects induced by DNA hypermethylation could be rescued by mutant alleles of the histone H3-K9 methyltransferase gene Su(var)3-9 PMID: 12640138
central role of the SU(VAR)3-9 protein in heterochromatic gene silencing PMID: 12723694
The full-length SU(VAR)3-9 enzyme transfers two methyl groups to lysine 9 within a histone 3 tail peptide in a nonprocessive manner, requiring the N-terminus for homodimerization and for full activity in vitro. PMID: 15035645
displays extensive H3-K9 di- and trimethylation within but also outside pericentric heterochromatin PMID: 15574598
Saturation transfer difference NMR measurements were performed to investigate the interaction of S-adenosyl-l-methionine (AdoMet) with SU(VAR)3-9 PMID: 15835908
A mutation in SuUR gene results in a decrease in the amount of SU(VAR)3-9 binding sites in autosomes.the SU(VAR)3-9 protein was found in pericentric heterochromatin and at 223 sites on euchromatic arms. PMID: 16607511
SU(VAR)3-9, facilitates HP1 binding to chromatin methylated at lysine 9 within the H3 N terminus (H3K9). PMID: 17101786
describe how, in double mutants for Su(var)3-9 and Suppressor of Under-Replication genes encoding two structural proteins of heterochromatin, new banded heterochromatic segments appear in all polytene chromosomes PMID: 17640911

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Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EIF2G subfamily

Database Links

KEGG: dme:Dmel_CG43665

STRING: 7227.FBpp0302537

UniGene: Dm.3299

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Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 2 subunit 3 (Su (var)3-9)

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