Recombinant Drosophila melanogaster Fat-like cadherin-related tumor suppressor homolog (kug), partial

Code CSB-YP896159DLU
MSDS
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Source Yeast
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Code CSB-EP896159DLU
MSDS
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Source E.coli
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Code CSB-EP896159DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896159DLU
MSDS
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Source Baculovirus
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Code CSB-MP896159DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
kug
Uniprot No.
Alternative Names
kug; fat2; CG7749; Fat-like cadherin-related tumor suppressor homolog; Protein kugelei
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the planar polarity of actin filament orientation at the basal side of ovarian follicle cells. Required for proper egg chamber shape and elongation of the egg chamber during oogenesis. Required for the correct planar polarization of Rab10 within the basal follicle cell epithelium and is therefore indirectly involved in the Rab10-dependent remodeling of the basal membrane during egg chamber elongation.
Gene References into Functions
  1. Data show that the atypical cadherin Fat2 directs and orchestrates transmission of the intracellular actomyosin asymmetry cue onto a tissue plane and facilitate epithelial rotation in the opposite direction. PMID: 29176774
  2. Concordant microtubule polarity, but not microtubule alignment, requires the atypical cadherin Fat2, which acts at an early stage to translate plus-end bias into coordinated actin-mediated collective cell migration. PMID: 27134170
  3. Planar polarized localization of Fat2 depends on intact microtubules.Microtubule polarity depends on the atypical cadherin Fat2. PMID: 23831293
  4. Fat2 is required for the planar polarity of actin filament orientation at the basal side of ovarian follicle cells PMID: 19906848
  5. fat-like gene of Drosophila is involved in the formation of tubular organs PMID: 15047711

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Note=Accumulates on cell membranes where the planar oriented actin filaments terminate.
Tissue Specificity
Localizes where basal actin filaments terminate.
Database Links

KEGG: dme:Dmel_CG7749

STRING: 7227.FBpp0304968

UniGene: Dm.3778

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