Recombinant Drosophila melanogaster Flap endonuclease GEN (Gen), partial

Code CSB-YP894726DLU
MSDS
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Source Yeast
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Code CSB-EP894726DLU
MSDS
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Source E.coli
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Code CSB-EP894726DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894726DLU
MSDS
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Source Baculovirus
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Code CSB-MP894726DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gen
Uniprot No.
Alternative Names
Gen; CG10670Flap endonuclease GEN; EC 3.1.-.-; Flap structure-specific endonuclease GEN; Xpg-like endonuclease; DmGEN
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded. Also possesses weak 5'- to 3'-exonuclease activity on nicked but not gapped double-stranded DNA. Does not cleave bubble-like or Holliday junction substrates.
Gene References into Functions
  1. the in vitro activities of DmGen and HsGEN1 are strikingly similar. PMID: 28369583
  2. Cloning and characterization of DmGEN, Drosophila Melanogaster XPG-like endonuclease, a RAD2 nuclease family member. PMID: 15576351
  3. DmGEN belongs to a new class (class IV) of the RAD2/XPG nuclease family PMID: 17614965
Subcellular Location
Nucleus.
Protein Families
XPG/RAD2 endonuclease family, GEN subfamily
Database Links

KEGG: dme:Dmel_CG10670

STRING: 7227.FBpp0076879

UniGene: Dm.847

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