Recombinant Drosophila melanogaster G1/S-specific cyclin-E (CycE), partial

Code CSB-YP345801DLU
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Source Yeast
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Code CSB-EP345801DLU
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Source E.coli
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Code CSB-EP345801DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345801DLU
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Source Baculovirus
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Code CSB-MP345801DLU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CycE; CG3938; G1/S-specific cyclin-E; DmCycE
Drosophila melanogaster (Fruit fly)
Protein Length
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Essential for the control of the cell cycle at the G1/S (start) transition. Targeted by archipelago for degradation by the SFC ubiquitin ligase complex.
Gene References into Functions
  1. These findings reveal a Gcm-Apt-CycE axis that regulates neuroblast and glia cell differentiation. PMID: 29625112
  2. we provide genetic evidences, that the Ama-Nrt-Abl-pathway regulates CycE expression by altering the function of the Hippo effector Yorkie in embryonic NBs PMID: 27015425
  3. We further detected that reduced level of GRK2 induced a small cell cycle arrest at G2/M phase by enhancing the expression of cyclin A, B1, and E PMID: 23460259
  4. CycE and Cdk2 are required for germline stem cell proliferation. PMID: 23293285
  5. p53-mediated regulation of ectopic neural stem cells (NSC) self-renewal through the Ago/Cyc E axis becomes particularly important when NSC homeostasis is perturbed. PMID: 22140513
  6. analysis of Abdominal-A mediated repression of Cyclin E expression during cell-fate specification in the Drosophila central nervous system PMID: 19799999
  7. The chromatin-remodeling protein Osa interacts with CyclinE in Drosophila eye imaginal discs. PMID: 20008573
  8. This work provides in vivo genetic evidence for p53-mediated integration of metabolic stress signals, which modulate the activity of the ubiquitin-proteasome system to degrade cyclin E protein and thereby impose cell cycle arrest. PMID: 20176921
  9. CycE inhibits the function of Prospero and facilitates its cortical localization, which is critical for inducing stem cell behaviour. PMID: 19914234
  10. follicular stem cell function was substantially restored by expressing either excess DE-cadherin or excess E2F1/DP, the transcription factor normally activated by CycE-Cdk2 phosphorylation of retinoblastoma proteins PMID: 19966222
  11. Brm complex mutants suppress the DmcycE(JP) phenotype by increasing S phases w/o affecting DmcycE protein levels. DmcycE physically interacts with Brm & Snr1 in vivo. The Brm complex inhibits S phase by acting downstream of DmcycE protein accumulation. PMID: 12093739
  12. centrosome overduplication still occurs in skpA-; cycE- mutant animals, demonstrating that high cyclin E levels are not necessary for centrosome overduplication PMID: 12730292
  13. we link the CSN to the degradation of Cyclin E, which promotes the G1-S transition in the cell cycle and then is rapidly degraded by the ubiquitin-proteasome pathway PMID: 12737805
  14. asymmetric divisions are due to upregulation of Cyclin E in late ganglion mother cells PMID: 14973280
  15. phosphorylation and degradation of Dup protein at G1/S requires cyclin E/CDK2 PMID: 15342466
  16. string(cdc25) and cyclin E control patterned histone expression at different stages of Drosophila embryonic development. PMID: 15355790
  17. critical role for CycE in regulating cell fate in segment-specific neural lineages. PMID: 15580266
  18. cyclin E functions downstream of the homeotic genes largly independently of its role in proliferation PMID: 15632940
  19. Cell cycle diversity involves differential regulation of Cyclin E activity in the Drosophila bristle cell lineage. PMID: 15829522
  20. The most significant cyclin E alteration is post-translational cleavage of full-length cyclin E into low molecular weight forms that are hyperactive compared to the 50-kDa, full-length protein and correlate with increasing stage of breast cancer. PMID: 16637514
  21. Fondue is a major hemolymph protein required for efficient clotting in Drosophila PMID: 16690050
  22. APC/C-Fzr activity is periodically inhibited by CycE-Cdk2, to prevent relicensing in S-phase cells. PMID: 18559483
  23. Minus contributes to cell cycle regulation in part by selectively controlling turnover of Cyclin E PMID: 19723762

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Subcellular Location
Protein Families
Cyclin family, Cyclin E subfamily
Tissue Specificity
Isoform II is ubiquitous in early embryos and, prior to mitosis 14, is rapidly degraded in all cells except the pole (germ) cells. Expressed during G1 phase in proliferating peripheral nervous system cells. Constitutive expression in embryonic cycles lack
Database Links

KEGG: dme:Dmel_CG3938

STRING: 7227.FBpp0294012

UniGene: Dm.3509

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