Recombinant Drosophila melanogaster GATA-binding factor A (pnr), partial

Code CSB-YP347094DLU
MSDS
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Source Yeast
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Code CSB-EP347094DLU
MSDS
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Source E.coli
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Code CSB-EP347094DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347094DLU
MSDS
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Source Baculovirus
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Code CSB-MP347094DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pnr
Uniprot No.
Alternative Names
pnr; GATA-A; CG3978; GATA-binding factor A; Protein pannier; Transcription factor GATA-A; dGATA-A
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator involved in several developmental processes during embryonic and imaginal disks development. Involved in determining dorsal cell fate. Acts as an essential transcriptional regulator of proneural achaete-scute complex (AS-C) and is required for its spatial regulation during development of the adult peripheral nervous system, and hence for the positioning of neural precursors. It is the only factor to directly activate AS-C genes.
Gene References into Functions
  1. pnr appears to be highly regulated by Dpp and Brk on both the DV and AP axes during embryo patterning. PMID: 22513375
  2. Stat and Pnr represent two essential factors controlling blood cell maturation in the developing lymph gland and exert their functions both in a cell-autonomous and non-cell-autonomous manner. PMID: 21295568
  3. Dorsal eye selector pannier (pnr) suppresses the eye fate to define dorsal margin of the Drosophila eye PMID: 20691679
  4. manipulation of pannier activity in either germ layer affects cardiac specification, suggesting that its function is required in both the mesoderm and the ectoderm PMID: 12756184
  5. Pnr is positevely regulated by Tou during neuronal development. PMID: 16141224
  6. show that the dual function of pnr during regulation of wingless is achieved by one isoform repressing expression of the morphogen in the dorsal-most region of the disc while the other laterally promotes activation of the notal wingless expression PMID: 18042352
  7. Pnr is essential for early cardiac progenitor formation and also plays an important role in maintaining proper heart function. PMID: 19494035

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG3978

STRING: 7227.FBpp0082675

UniGene: Dm.7432

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