Recombinant Drosophila melanogaster General odorant-binding protein lush (lush)

Code CSB-YP517298DLU
MSDS
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Source Yeast
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Code CSB-EP517298DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517298DLU
MSDS
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Source Baculovirus
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Code CSB-MP517298DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lush
Uniprot No.
Alternative Names
lush; Obp76a; Obp76c; CG8807; General odorant-binding protein lush
Species
Drosophila melanogaster (Fruit fly)
Expression Region
30-153
Target Protein Sequence
M TMEQFLTSLD MIRSGCAPKF KLKTEDLDRL RVGDFNFPPS QDLMCYTKCV SLMAGTVNKK GEFNAPKALA QLPHLVPPEM MEMSRKSVEA CRDTHKQFKE SCERVYQTAK CFSENADGQF MWP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Odorant-binding protein required for olfactory behavior and for activity of pheromone-sensitive neurons. Binds to alcohols and mediates avoidance behavior to high concentrations of alcohols, the alcohol-binding possibly resulting in activation of receptors on T2B neurons, the activation of these receptors inhibiting these neurons. Acts in concert with Snmp and lush to capture cVA molecules on the surface of Or67d expressing olfactory dendrites and facilitate their transfer to the odorant-receptor Orco complex. Required for cVA response, probably by binding to VA. May act by serving as an adapter that bridges the presence of gaseous pheromone molecules, cVA, to activation of specific neuronal receptors expressed on T1 olfactory neurons, possibly via a specific conformational change induced by cVA that in turn activates T1 receptors. T1 neurons are excited by the pheromone VA, while T2 neurons are inhibited by alcohols. Also binds to phthalates.
Gene References into Functions
  1. the ligand-binding cavity of LUSH protein can accommodate a variety ligands and pheromones with strong binding interactions PMID: 23121132
  2. the alcohol binding in the LUSH protein is the consequence of three important factors: H-bonding, solvation, and the other noncovalent interaction. PMID: 20550105
  3. Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster PMID: 12881720
  4. LUSH, a soluble odorant-binding protein, undergoes a major conformational change, triggered by ligands. PMID: 14759510
  5. genetic interaction between lush and spontaneous activity in 11-cis vaccenyl acetate-sensitive neurons in the absence of pheromone PMID: 15664171
  6. Detection of odorants by olfactory neurons is results from direct activation of odorant receptors by odor molecules; study shows that detection of the 11-cis vaccenyl acetate (cVA), is instead mediated by pheromone-induced conformational shifts in LUSH. PMID: 18585358

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Subcellular Location
Secreted.
Protein Families
PBP/GOBP family
Tissue Specificity
Specifically expressed in chemosensory system in both males and females. Expressed in a subset of trichoid chemosensory sensilla located on the ventral-lateral surface of the third antennal segment. Secreted from non-neuronal support cells into the sensil
Database Links

KEGG: dme:Dmel_CG8807

STRING: 7227.FBpp0074737

UniGene: Dm.2522

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