Recombinant Drosophila melanogaster Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GalTA), partial

Code CSB-YP748703DLU
MSDS
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Source Yeast
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Code CSB-EP748703DLU
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Source E.coli
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Code CSB-EP748703DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP748703DLU
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Source Baculovirus
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Code CSB-MP748703DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
C1GalTA
Uniprot No.
Alternative Names
C1GalTA; CG9520; Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; EC 2.4.1.122; Core 1 O-glycan T-synthase; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1; Core 1 beta1,3-galactosyltransferase 1; C1GalT1; Core 1 beta3-Gal-T1
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins.
Gene References into Functions
  1. T3lec, but not T2lec and T4lec, had a clear activating effect on Drosophila melanogaster core 1 galactosyltransferase enzyme activity PMID: 27738109
  2. named the CG9520 gene Drosophila core 1 beta1,3-galactosyltransferase 1 because it is responsible for the synthesis and function of T antigen in vivo. PMID: 18824562
  3. Lectin staining and blotting experiments confirmed that core 1 galactosyltransferase contributes to the synthesis of Gal-beta1,3-GalNAc in vivo PMID: 18985719
Subcellular Location
Membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 31 family, Beta3-Gal-T subfamily
Tissue Specificity
In stage 12 embryos, expression is seen in the amnioserosa and by stage 16, also in the central nervous system, predominantly the ventral nerve cord and brain. Also in embryos, preferential expression is seen in the ejaculatory duct and the nurse cells (b
Database Links

KEGG: dme:Dmel_CG9520

STRING: 7227.FBpp0079388

UniGene: Dm.366

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