Recombinant Drosophila melanogaster Gram-negative bacteria-binding protein 1 (GNBP1)

Code CSB-YP873594DLU
MSDS
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Source Yeast
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Code CSB-EP873594DLU
MSDS
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Source E.coli
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Code CSB-EP873594DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873594DLU
MSDS
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Source Baculovirus
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Code CSB-MP873594DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GNBP1
Uniprot No.
Alternative Names
GNBP1; CG6895Gram-negative bacteria-binding protein 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
20-494
Target Protein Sequence
Y KIPTPTVELL ETGFSVSIPD EEGVKVVAFN VNRNRNFTSF INEGQYNVRL TEPQNGRWTT NFSSVPLRSQ DVLYLWTSVQ HQKAVYQDLA QPLPVCNLGG EYRPRGCSPG DDDFTDDNQL STEDSALEPT APSVCEPSES QVSPQIGVSI CKGQLLFEET FDQLNESLWI HDVRLPLDSK DAEFVLYDGK AKVHDGNLVI EPLLWSSYRP DLSIANSRLD LSERCTGTHN RIKECILHST GSGPSGIMPP IVTPRISTKE TFAFQYGRIE IRAKLPKGDW IVPLLLLEPL TEWYGQSGYE SGQLRVALAR GNSVLRMPRG KLVDGRSLYG GPVLSTDAHQ REDLWLSKRK ISHFGDDFHT YSLDWSSNRL LFSVDGQVYG EMLNGFTELD ENPRWKQGGP MAPFDKMFYI SLGVSVGGFG DFVDHLRTAT YEKPWANYHP QAKLQFHQAQ DQWLPTWKQP ALKIDYVRVF ATDN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a key role in innate immunity by acting as a pattern recognition receptor for beta-1,3-glucan from fungi and lipopolysaccharide from Gram-negative bacteria. Upon recognition of invading microorganism-derived products, acts upstream of protease spz processing enzyme SPE to activate the Toll pathway and to induce the expression of antimicrobial peptides drosomycin, cecropin and attacin.
Gene References into Functions
  1. GNBP1 acts upstream of the Toll ligand Spatzle and works in the defense against Gram-positive bacterial infection in Drosophila PMID: 14722090
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Note=Secreted and attached to the membrane by a GPI-anchor.
Protein Families
Insect beta-1,3-glucan binding protein family
Database Links

KEGG: dme:Dmel_CG6895

STRING: 7227.FBpp0074817

UniGene: Dm.2810

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