Recombinant Drosophila melanogaster Gram-negative bacteria-binding protein 3 (GNBP3)

Code CSB-YP885665DLU
MSDS
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Source Yeast
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Code CSB-EP885665DLU
MSDS
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Source E.coli
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Code CSB-EP885665DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885665DLU
MSDS
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Source Baculovirus
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Code CSB-MP885665DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GNBP3
Uniprot No.
Alternative Names
GNBP3; CG5008Gram-negative bacteria-binding protein 3
Species
Drosophila melanogaster (Fruit fly)
Expression Region
26-490
Target Protein Sequence
YEVPK AKIDVFYPKG FEVSIPDEEG ITLFAFHGKL NEEMEGLEAG TWARDIVKAK NGRWTFRDRI TALKPGDTLY YWTYVIYNGL GYREDDGSFV VNGYSGNNAS PHPPVVPVST TPWTPPADPD IDIRLGCTTP KTEVNGAPTR CAGQLVFVDE FNAAKLDPNK WKAERRFSGQ PDYEFNVYVD DAPETLCLAN GHVVLSTNTM KKQFKKGSGE SLDLGEKCTG QANTHDCVRN GRTLNDGLPP MVTAQFSSKD FSFKYGRVEV RAKMPRAQWV TPQIWLQPRR PIYGVDDYRS GQLRIAYTRP NGGNLDLYGA AVLFADEPLR SVKNCLKPGT GNNSEDWSDS FHNYTLEWTP RELRWLVDGK EWCVQGSAKG SFSETTAAGK SLPQAQKLEE GTGLAPFDQE FYLTFGLSVG GFNEYQHEIK PWNERAPQAQ KAFWKEVKKI RDHWLDEGHM KIDYVKVYSL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the recognition of invading microorganisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.
Gene References into Functions
  1. Data show that Spn1 is an active protease inhibitor of the serpin superfamily, and support a role of Spn1 as a repressor of GNBP3-dependent Toll activation upon fungal infection. PMID: 21576362
  2. GNBP3 agglutinates fungal cells & activates melanization in a Toll-independent manner. GNBP3 assembles "attack complexes", which comprise phenoloxidase and the necrotic serpin. PMID: 20201042
  3. Here, we report that GNBP3 is a pattern recognition receptor that is required for the detection of fungal cell wall components. Strikingly, we find that there is a second, parallel pathway acting jointly with GNBP3. PMID: 17190605
  4. N-terminal domain of Drosophila Gram-negative binding protein 3 (GNBP3) has a role in beta-glucan recognition PMID: 19692333
  5. GNBP3-N terminal crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 134.79, b = 30.55, c = 51.73 A, beta = 107.4 degrees, and diffracted to 1.7 A using synchrotron radiation. PMID: 19724120

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Subcellular Location
Secreted.
Protein Families
Insect beta-1,3-glucan binding protein family
Database Links

KEGG: dme:Dmel_CG5008

STRING: 7227.FBpp0076237

UniGene: Dm.898

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