Recombinant Drosophila melanogaster Histone-like protein 18C (Mst77F)

Code CSB-YP323476DLU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP323476DLU
MSDS
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Source E.coli
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Code CSB-EP323476DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323476DLU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP323476DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mst77F
Uniprot No.
Alternative Names
Mst77F; 18c; anon-77F; CG3354Histone-like protein 18C
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-215
Target Protein Sequence
MSNLKQKDSK PEVAVTKSVK TYKKSIEYVN SDASDIEEDI NRAEDEYASS SGFVNFLRDF KKRYGEYYSN NEIRRAAETR WNEMSFRHRC QYSAEPLDTF HVEPNSVSSL QRSSEGEHRM HSEISGCADT FFGAGGSNSC TPRKENKCSK PRVRKSCPKP RAKTSKQRRS CGKPKPKGAR PRKACPRPRK KMECGKAKAK PRCLKPKSSK PKCSM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Not known. Encoded in the intron of cAMP-dependent protein kinase regulatory chain type I.
Gene References into Functions
  1. Data demonstrate that Mst77F is required for the proper compaction of spermatid chromatin following the histone-to-protamine transition, and show that Mst77F is proteolytically processed during spermiogenesis, in a way remarkably similar to mammalian P2. PMID: 27810970
  2. An intrinsically unstructured domain in the C-terminus of Mst77F binds DNA via electrostatic interaction. This binding results in structural reorganization of the domain, which induces interaction with an N-terminal region of the protein. PMID: 25735749
  3. Spermatids of ms(3)nc3 (Mst77F(1)) mutants show disturbed nuclear shaping, instability of perinuclear microtubules but no obvious chromatin condensation defects. PMID: 20138392
  4. identification of functional Mst77Y genes PMID: 19897751
Database Links

KEGG: dme:Dmel_CG3354

STRING: 7227.FBpp0077928

UniGene: Dm.2510

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