Recombinant Drosophila melanogaster Homeobox protein cut (ct), partial

Code CSB-YP320723DLU
MSDS
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Source Yeast
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Code CSB-EP320723DLU
MSDS
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Source E.coli
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Code CSB-EP320723DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP320723DLU
MSDS
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Source Baculovirus
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Code CSB-MP320723DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ct
Uniprot No.
Alternative Names
ct; CG11387Homeobox protein cut
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of cell fate decisions in multiple lineages. Specifically, functions as a determination factor that specifies sensory organ identity in precursor cells. Probably also involved in cell type specification of Malpighian tubules. In absence of cut gene external sensory organs are transformed into chordotonal organs.
Gene References into Functions
  1. These analyses identified a host of putative Cut and/or Knot effector molecules, and a subset of these putative TF targets converge on modulating dendritic cytoskeletal architecture, which are grouped into three major phenotypic categories, based upon neuromorphometric analyses: complexity enhancer, complexity shifter, and complexity suppressor PMID: 29025914
  2. Drosophila transcription factor Cut coordinately controls differentiation and repression of apoptosis via direct regulation of the pro-apoptotic gene PMID: 22438831
  3. Turtle is downstream effector of Cut-mediated regulation of da neuron dendrite morphology. PMID: 21811639
  4. Study of distant interactions of LCR from the locus CUT. PMID: 20886739
  5. bft acts downstream of cut and tramtrack to implement correct bristle morphogenesis PMID: 12019237
  6. Gene loci that interact with cut during Drosophila wing-margin development were identified. PMID: 15956666
  7. Cut functions in regulating both cell differentiation and the cell cycle, and downregulation of Cut by Notch contributes to the mitotic cycle/endocycle switch and cell differentiation in follicle cells. PMID: 16141223
  8. These results indicate that Cut functions as a transcriptional repressor of dPCNA gene by binding to the promoter region in the differentiated state PMID: 16629902
  9. cut control the dendrite cytoskeleton and synergize to define final arbor shape. PMID: 18093520
  10. cut protein acts in parallel to atonal, spalt-major and spalt-related transcription factors for differentiation of Johnston's organ of Drosophila antennae. PMID: 18820445

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Subcellular Location
Nucleus.
Protein Families
CUT homeobox family
Tissue Specificity
Detected in many cells in the central nervous system, all external sensory organs, some peripheral neurons, and in the non-neural cells of the spiracles and the Malpighian tubules.
Database Links

KEGG: dme:Dmel_CG11387

STRING: 7227.FBpp0113105

UniGene: Dm.1827

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