Recombinant Drosophila melanogaster Homeobox protein extradenticle (exd)

Code CSB-YP328114DLU
MSDS
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Source Yeast
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Code CSB-EP328114DLU
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Source E.coli
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Code CSB-EP328114DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328114DLU
MSDS
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Source Baculovirus
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Code CSB-MP328114DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
exd
Uniprot No.
Alternative Names
exd; CG8933Homeobox protein extradenticle; Dpbx
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-376
Target Protein Sequence
MEDPNRMLAH TGGMMAPQGY GLSGQDDGQN AGSENEVRKQ KDIGEILQQI MSISEQSLDE AQARKHTLNC HRMKPALFSV LCEIKEKTVL SIRNTQEEEP PDPQLMRLDN MLIAEGVAGP EKGGGGAAAA SAAAASQGGS LSIDGADNAI EHSDYRAKLA QIRQIYHQEL EKYEQACNEF TTHVMNLLRE QSRTRPITPK EIERMVQIIH KKFSSIQMQL KQSTCEAVMI LRSRFLDARR KRRNFSKQAS EILNEYFYSH LSNPYPSEEA KEELARKCGI TVSQVSNWFG NKRIRYKKNI GKAQEEANLY AAKKAAGASP YSMAGPPSGT TTPMMSPAPP QDSMGYPMGS GGYDQQQPYD NSMGGYDPNL HQDLSP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor which acts with the selector homeodomain proteins altering the regulation of downstream target genes such as wingless (wg), teashirt (tsh) and decapentaplegic (dpp), thus affecting segmental identity. Delimits the eye field and prevent inappropriate eye development. Required for proper localization of chordotonal organs within the peripheral nervous system.
Gene References into Functions
  1. Hth functions together with its co-factor Extradenticle to repress the R8-specific factor Senseless in dorsal rim area R8 cells, allowing expression of an ultraviolet-sensitive R7 Rhodopsin. PMID: 24496628
  2. cooperative binding sites for En, with the homeodomain-containing Hox cofactors Extradenticle (Exd) and Homothorax (Hth) was localized, within two CRMs that drive similar expression patterns PMID: 22537495
  3. In the flight muscles, exd and hth are genetically upstream of another muscle identity gene, salm, and are direct transcriptional regulators of the signature flight muscle structural gene, Actin88F. PMID: 22975331
  4. Using computational platform, SELEX-seq, study shows that all eight Drosophila Hox proteins obtain novel recognition properties when they bind DNA with the dimeric cofactor Extradenticle-Homothorax. PMID: 22153072
  5. Homothorax and extradenticle control dendritic and axonal targeting of olfactory projection neurons in the Drosophila brain. PMID: 21801717
  6. Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity. PMID: 21262810
  7. Hox repression of a target gene: extradenticle-independent, additive action through multiple monomer binding sites. PMID: 12070087
  8. in thoracic segments, Exd is required for repression (as well as activation) by En. This is consistent with in vitro results showing that Exd is involved in recognition of positive and negative targets. PMID: 12506003
  9. we show that the functions of both of the Hox cofactors Extradenticle and Homothorax play essential roles in repression by Engrailed. PMID: 12506004
  10. Labial, Ultrabithorax, Antennapedia, Extradenticle and Homothorax are regulated in a signal transduction pathway in developing Drosophila brain PMID: 15172684
  11. homothorax/extradenticle is repressed in the body regions where eyg is active PMID: 15634705
  12. distinct Hox/Exd/Hth complexes select different binding sites, and a given complex recognizes a consensus DNA sequence PMID: 15753213
  13. Extradenticle and Homothorax selector proteins control branchless/FGF expression in mesodermal bridge-cells PMID: 16007069
  14. nuclear EXD is more extensively phosphorylated than the cytoplasmic form, suggesting that EXD is a target of signal transduction by protein kinases PMID: 16403493
  15. Exd contains an NLS mask that contributes to its cytoplasmic localization. PMID: 17277370
  16. This finding highlights that the Hox protein Ubx has multiple ways to interact with the Exd cofactor and suggests that flexibility in Hox-PBC contacts contributes to specify and diversify Hox protein function. PMID: 17942685

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Subcellular Location
Nucleus. Note=Nuclear translocation requires interaction with hth.
Protein Families
TALE/PBX homeobox family
Tissue Specificity
Prior to full germband retraction it is ubiquitously present, after germband retraction, mostly present in the anterior portion of the ventral nerve cord.
Database Links

KEGG: dme:Dmel_CG8933

STRING: 7227.FBpp0073940

UniGene: Dm.174

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