Product Details

Purity

>85% (SDS-PAGE)

Target Names

cad

Uniprot No.

P09085

Alternative Names

cad; CG1759Homeotic protein caudal

Species

Drosophila melanogaster (Fruit fly)

Expression Region

1-427

Target Protein Sequence

MVSHYYNTLP YTQKHSAANL AYASAAGQPW NWTPNYHHTP PNHQFLGDVD SSHAAHHAAA AHQMYYNSHH MFHSAAAASA GEWHSPASST ADNFVQNVPT SAHQLMQQHH HHHAHASSSS ASSGSSSSGG APGAPQLNET NSSIGVGGAG GGGGVGGATD GGPGSAPPNH QQHIAEGLPS PPITVSGSEI SSPGAPTSAS SPHHHLAHHL SAVANNNNNN NNNNNSPSTH NNNNNNNSVS NNNRTSPSKP PYFDWMKKPA YPAQPQPGKT RTKDKYRVVY TDFQRLELEK EYCTSRYITI RRKSELAQTL SLSERQVKIW FQNRRAKERK QNKKGSDPNV MGVGVQHADY SQLLDAKAKL EPGLHLSHSL AHSMNPMAAM NIPAMRLHPH LAAHSHSLAA VAAHSHQLQQ QHSAQMSAAA AVGTLSM

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Caudal (cad) is one of a number of transcription factors controlling segmentation of the embryo. Further transcriptional regulation via a 5' flanking region containing DNA replication-related elements (DRE) and by dref also regulated by trh and tgo via the CNS midline element. Alongside Bicoid (bcd), caudal forms concentration gradients down the anterior-posterior (A-P) axis providing positional information and subsequent induction of the gap genes. Plays a role in gastrulation/germ band extension, hindgut morphogenesis, positive regulation of cell proliferation, genital disk development and pattern formation. Acts as a key regulator of the Hox gene network and activates transcription via the downstream core promoter element (DPE) relative to the TATA box. Plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets including fog, fkh and wg. Caudal is involved in regulation of proliferation through transactivation of the E2F gene. Postembryonically its function is mostly restricted to the intestine where it regulates antimicrobial peptide (AMP) levels preserving the normal gut flora.

Gene References into Functions

In this study, ChIP/chip datasets are analyzed using the corresponding PWMs for the well-studied TFs; CAUDAL, HUNCHBACK, KNIRPS and KRUPPEL, to determine the distribution of predicted binding sites. All four TFs are critical regulators of gene expression along the anterio-posterior axis in early Drosophila development PMID: 27264535
Using a systematic series of deletion mutants (all containing the intact DNA-binding homeodomain) we discovered that the C-terminal region of Caudal contributes to the preferential activation of the fushi tarazu (ftz) Caudal target gene. PMID: 26018075
Bicoid acts through a 63 nt response element in the caudal 3' untranslated region that includes a single miR-2 target site. Four predicted Bicoid splice isoforms are capable of caudal repression; all require the microRNA target for repression. PMID: 23910663
Abdominal-B and caudal inhibit the formation of specific neuroblasts in the Drosophila tail region. PMID: 23903193
Bicoid recruits Bin3 to the caudal 3' UTR. PMID: 21262214
Bicoid associates with the 5'-cap-bound complex of caudal mRNA and represses translation. PMID: 12368268
The caudal homeodomain protein activates Drosophila E2F gene expression. PMID: 12466526
In this study, we have identified the binding sites for bHLH-PAS proteins, referred to as CNS midline element (CME), in the 5'-flanking region of the Drosophila caudal gene. PMID: 17207870
study shows that the intestinal homeobox gene Caudal regulates the commensal-gut mutualism by repressing nuclear factor kappa B-dependent antimicrobial peptide genes PMID: 18218863
Age- and oxidative-stress-related upregulation of the caudal gene is mediated by the NF-kappaB binding site located in the 5'-flanking region of the caudal gene. PMID: 18656526

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Subcellular Location

Nucleus.

Protein Families

Caudal homeobox family

Tissue Specificity

Maternally localized in an anteroposterior gradient in the syncytial blastoderm. Also expressed in the pole cells. Zygotically localized in the primordia of the terminal abdominal segment, the hindgut and in the posterior midgut rudiment. Expressed in the

Database Links

KEGG: dme:Dmel_CG1759

STRING: 7227.FBpp0080942

UniGene: Dm.2073

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Recombinant Drosophila melanogaster Homeotic protein caudal (cad)

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