Recombinant Drosophila melanogaster Integrin beta-nu (betaInt-nu), partial

Code CSB-YP632932DLU
MSDS
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Source Yeast
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Code CSB-EP632932DLU
MSDS
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Source E.coli
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Code CSB-EP632932DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP632932DLU
MSDS
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Source Baculovirus
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Code CSB-MP632932DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Itgbn
Uniprot No.
Alternative Names
Itgbn; beta-nu; betaInt-nu; Itgbetanu; CG1762; Integrin beta-nu
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Contributes to endodermal integrity and adhesion between the midgut epithelium and the surrounding visceral muscle. Essential for migration of the primordial midgut cells and for maintaining, but not establishing, cell polarity in the midgut epithelium. Can only partially compensate for the loss of beta-PS integrin during primordial midgut cell migration. The two beta subunits mediate midgut migration by distinct mechanisms: beta-PS requires rhea/Talin and beta-nu does not. Integrin alpha-PS3/beta-nu is required for effective phagocytosis of apoptotic cells during embryonic development and for the phagocytic elimination of S.aureus by mediating the binding of S.aureus peptidoglycan to larval hemocytes, which probably activates a signaling pathway involving Rac1 and Rac2. Not required for the production of antimicrobial peptides during S.aureus. Upon activation by LanA, integrin alpha-PS3/beta-nu activates Fak in presynapsis to suppress neuromuscular junction (NMJ) growth during larval development and during low crawling activity, but not during higher-crawling conditions. Mediates, together with LanA, glutamate receptor-modulated NMJ growth.
Gene References into Functions
  1. that integrin alphaPS3/betanu serves as a receptor in the phagocytosis of apoptotic cells and bacteria by Drosophila phagocytes. PMID: 23426364
  2. Draper and integrin betanu cooperate in the phagocytic elimination of S. aureus by recognizing distinct cell wall components PMID: 22547074
  3. Mutagenesis of the integrin cytoplasmic domain, within the chimera, showed the dominant-negative inhibition was not due to talin sequestration alone and that additional interactions are required. PMID: 16569666
Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Integrin beta chain family
Tissue Specificity
Expression is confined to the developing midgut endoderm and its precursors during embryogenesis. In the larvae, expression is concentrated in the midgut imaginal disks. Expressed in embryonic and larval hemocytes (at protein level).
Database Links

KEGG: dme:Dmel_CG1762

STRING: 7227.FBpp0080990

UniGene: Dm.19859

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