Recombinant Drosophila melanogaster Interference hedgehog (ihog), partial

Code CSB-YP895632DLU
MSDS
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Source Yeast
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Code CSB-EP895632DLU
MSDS
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Source E.coli
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Code CSB-EP895632DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895632DLU
MSDS
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Source Baculovirus
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Code CSB-MP895632DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ihog
Uniprot No.
Alternative Names
ihog; CG9211Interference hedgehog
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates response to the active Hedgehog (Hh) protein signal in embryos, functioning upstream or at the level of patched (ptc).
Gene References into Functions
  1. Hh and its co-receptor Ihog are in exovesicles transported via cytonemes. Hh transport via exosomes along cytonemes is a significant mechanism for the restricted distribution of a lipid-modified morphogen. PMID: 25472772
  2. A functional interaction between Ihog and members of the Shf/WIF1 family demonstrates that Ihog can increase the Wnt-inhibitory activity of vertebrate WIF1. PMID: 23154411
  3. Data show that Dispatched could be involved in the regulation of vesicular trafficking necessary for basolateral release of Hh, Dlp, and Ihog. PMID: 21690386
  4. Results describe the interaction of interference hedgehog with Hedgehog ligands via a specific fibronectin III domain. PMID: 16630821
  5. Hedgehog directly binds Ihog and provide the first demonstration of a specific role for heparin in Hedgehog responsiveness PMID: 17077139

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily, IHOG family
Database Links

KEGG: dme:Dmel_CG9211

STRING: 7227.FBpp0078993

UniGene: Dm.11441

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