Recombinant Drosophila melanogaster Kinesin-like protein costa (cos), partial

Code CSB-YP522704DLU
MSDS
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Source Yeast
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Code CSB-EP522704DLU
MSDS
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Source E.coli
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Code CSB-EP522704DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522704DLU
MSDS
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Source Baculovirus
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Code CSB-MP522704DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cos
Uniprot No.
Alternative Names
cos; cos2; costal-2; CG1708Kinesin-like protein costa; Kinesin-like protein costal2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates cubitus interruptus (ci) processing by recruiting multiple kinases to promote its efficient phosphorylation. Scaffolds multiple kinases and ci into proximity to promote its hyperphosphorylation, which then targets it for SCFSlimb/proteasome-mediated processing to generate its repressor form. Hh signaling inhibits ci phosphorylation by interfering with the cos-ci-kinases complex formation. Negatively regulates hh-signaling pathways during various processes, including photoreceptor differentiation. May negatively regulate a hh-signaling pathway which functions in the intestinal immune response to bacterial uracil by activating the Duox-dependent production of reactive oxygen species (ROS).
Gene References into Functions
  1. Cos2 is sumoylated, which counteracts its inhibitory role on Smo accumulation in the wing. PMID: 28195188
  2. Data show Ci interaction with Su(fu) or Cos2 correlates with Su(fu)- or Cos2-mediated suppression of Ci activity PMID: 26271100
  3. The CORD region of Ci has a second function distinct from Cos2 binding that inhibits Ci processing. Cos2 binding to CORD relieves this inhibition. PMID: 20850429
  4. analysis of phosphorylation of the kinesin Costal-2 in vivo reveals requirement of fused kinase activity for all levels of hedgehog signalling PMID: 20435030
  5. Costal2 associates with membranes in a Hedgehog-sensitive, Smoothened-independent manner PMID: 14645371
  6. Cos2 recruits multiple kinases to efficiently phosphorylate Cubitus interruptus. PMID: 15691767
  7. Differential Hh target gene regulation can be accomplished by differential sensitivity of Cos2 and Su(Fu) to Hh. PMID: 15750186
  8. Cos2 motility plays an important role in its function, regulating the amounts and activity of Ci that ultimately interpret the level of Hh to which cells are exposed. PMID: 18691888

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KIF27 subfamily
Database Links

KEGG: dme:Dmel_CG1708

STRING: 7227.FBpp0088087

UniGene: Dm.5775

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