Recombinant Drosophila melanogaster Lipid storage droplets surface-binding protein 1 (Lsd-1)

Code CSB-YP894204DLU
MSDS
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Source Yeast
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Code CSB-EP894204DLU
MSDS
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Source E.coli
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Code CSB-EP894204DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894204DLU
MSDS
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Source Baculovirus
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Code CSB-MP894204DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lsd-1
Uniprot No.
Alternative Names
Lsd-1; CG10374Lipid storage droplets surface-binding protein 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-431
Target Protein Sequence
MATATSGSGL HLEAIDRIGS IPLVESSVKR VETIYDKVKN NNRLFSWYFE TAEATISAAY ETIQPAVKLF EPSIQRLDNV MCKSLDILEQ RIPLVYLPPE MMYWNTKEYM SDHLVRPVLK RADSVKQIGN AVLESPLTTY AAERIDGAFT VGDKFVDKYL VPIQTDQDQT DVKKLVSALN DNFSPIRQQL NRMFGHKSPQ EDDNEAVPDE RGAIKAIHHG QRFSRKLKRR LTQRTIAEAR ALKKQSKEAI HVLFYAAELI ATDPKQAVQK AKELWVYLSA DEPENQARPA TLEQLIVLLT RESARRVVHL VNFSAHVAAN IPRNLAHTTT EVAHHIIYIN HRIITISRLD KVKTISKEEA ESLFKRMLAF YGSLQGLTNA YLERVASFLS GRMEAEKVTG SDGGNSNHRS SRRRQDPNHY SATHNNINGV Y
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for normal deposition of neutral lipids in the oocyte.
Gene References into Functions
  1. Knockdown of the lipid storage droplet 1 gene in the entire Drosophila melanogaster body caused lethality, indicating that Lsd1 is an essential function for viability. PMID: 27136547
  2. Lsd1 restricts the number of GSC-like cells by regulating a diverse group of genes and provide further evidence that escort cell function must be carefully controlled during development and adulthood to ensure proper germline differentiation. PMID: 24625679
  3. SIRT1, in complex with LSD1, has a role in developmental epigenetic gene repression and regulation. PMID: 21596603
  4. Phosphorylation of Lsd1 promotes a 1.7-fold activation of the main fat body lipase and demonstrates the direct link between Lsd1 phosphorylation and activation of lipolysis. PMID: 18342616
Subcellular Location
Cytoplasm. Lipid droplet. Note=Cytoplasmic and in lipid storage droplets of adipocytes of first instar larvae. Colocalizes with Lsd-2 on the surface of neutral lipid storage droplets in cells of fat bodies.
Protein Families
Perilipin family
Database Links

KEGG: dme:Dmel_CG10374

STRING: 7227.FBpp0083846

UniGene: Dm.1300

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