Recombinant Drosophila melanogaster Maternal effect protein staufen (stau), partial

Code CSB-YP329523DLU
MSDS
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Source Yeast
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Code CSB-EP329523DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329523DLU
MSDS
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Source Baculovirus
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Code CSB-MP329523DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
stau
Uniprot No.
Alternative Names
stau; CG5753; Maternal effect protein staufen
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein which forms ribonucleoprotein complexes (RNPs) that play critical roles in the localization, translational repression and turnover of RNAs during embryogenesis, neurotransmission and neurogenesis. In the oocyte, essential for the localization of both the osk/oskar mRNA to the posterior pole and bcd/bicoid RNA to the anterior pole, and is therefore required for the correct anterior-posterior patterning of the developing embryo. Association with osk or bcd at their respective poles, appears to promote the formation and stabilization of the ribonucleoprotein complexes. Integral component of diverse neuritic ribonucleoprotein complexes (RNPs) that mediate the transport, translation and turnover of neuronal RNAs during neuorgenesis and the translation repression of synaptic transcripts in preparation for their dendritic targeting.
Gene References into Functions
  1. The role of Miranda in the localization of Staufen during the division of neuroblasts is described. PMID: 26423004
  2. Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number. PMID: 24951879
  3. establishment of developmental precision and proportions in the early Drosophila embryo PMID: 11845210
  4. role in long-term memory PMID: 12593794
  5. Miranda has a role in coupling oskar mRNA/Staufen complexes to the bicoid mRNA localization pathway PMID: 16905128

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Subcellular Location
Cytoplasm, Cytoplasmic ribonucleoprotein granule.
Tissue Specificity
Polar granules at the posterior pole of the oocyte, and by the time the egg is laid, at the anterior pole.
Database Links

KEGG: dme:Dmel_CG5753

STRING: 7227.FBpp0085962

UniGene: Dm.4722

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