Recombinant Drosophila melanogaster Maternal effect protein staufen (stau), partial

Code CSB-EP329523DLU
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
stau
Uniprot No.
Research Area
Others
Species
Drosophila melanogaster (Fruit fly)
Source
E.coli
Expression Region
321-582aa
Target Protein Sequence
RYNKITHQYRLTEERGPAHCKTFTVTLMLGDEEYSADGFKIKKAQHLAASKAIEETMYKHPPPKIRRSEEGGPMRTHITPTVELNALAMKLGQRTFYLLDPTQIPPTDSIVPPEFAGGHLLTAPGPGMPQPPPPPAYALRQRLGNGFVPIPSQPMHPHFFHGPGQRPFPPKFPSRFALPPPLGAHVHHGPNGPFPSVPTPPSKITLFVGKQKFVGIGRTLQQAKHDAAARALQVLKTQAISASEEALEDSMDEGDKKSPISQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
32.8
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
RNA-binding protein which forms ribonucleoprotein complexes (RNPs) that play critical roles in the localization, translational repression and turnover of RNAs during embryogenesis, neurotransmission and neurogenesis. In the oocyte, essential for the localization of both the osk/oskar mRNA to the posterior pole and bcd/bicoid RNA to the anterior pole, and is therefore required for the correct anterior-posterior patterning of the developing embryo. Association with osk or bcd at their respective poles, appears to promote the formation and stabilization of the ribonucleoprotein complexes. Integral component of diverse neuritic ribonucleoprotein complexes (RNPs) that mediate the transport, translation and turnover of neuronal RNAs during neuorgenesis and the translation repression of synaptic transcripts in preparation for their dendritic targeting.
Gene References into Functions
  1. The role of Miranda in the localization of Staufen during the division of neuroblasts is described. PMID: 26423004
  2. Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number. PMID: 24951879
  3. establishment of developmental precision and proportions in the early Drosophila embryo PMID: 11845210
  4. role in long-term memory PMID: 12593794
  5. Miranda has a role in coupling oskar mRNA/Staufen complexes to the bicoid mRNA localization pathway PMID: 16905128

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Subcellular Location
Cytoplasm, Cytoplasmic ribonucleoprotein granule.
Tissue Specificity
Polar granules at the posterior pole of the oocyte, and by the time the egg is laid, at the anterior pole.
Database Links

KEGG: dme:Dmel_CG5753

STRING: 7227.FBpp0085962

UniGene: Dm.4722

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