Recombinant Drosophila melanogaster Methyltransferase-like protein 14 homolog (CG7818)

Code CSB-YP896115DLU
MSDS
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Source Yeast
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Code CSB-EP896115DLU
MSDS
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Source E.coli
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Code CSB-EP896115DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896115DLU
MSDS
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Source Baculovirus
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Code CSB-MP896115DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CG7818
Uniprot No.
Alternative Names
Mettl14; KAR4; CG7818; N6-adenosine-methyltransferase non-catalytic subunit; Karyogamy protein 4; Methyltransferase-like protein 14; dMettl14
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-397
Target Protein Sequence
MSDVLKSSQE RSRKRRLLLA QTLGLSSVDD LKKALGNAED INSSRQLNSG GQREEEDGGA SSSKKTPNEI IYRDSSTFLK GTQSSNPHND YCQHFVDTGQ RPQNFIRDVG LADRFEEYPK LRELIKLKDK LIQDTASAPM YLKADLKSLD VKTLGAKFDV ILIEPPLEEY ARAAPSVATV GGAPRVFWNW DDILNLDVGE IAAHRSFVFL WCGSSEGLDM GRNCLKKWGF RRCEDICWIR TNINKPGHSK QLEPKAVFQR TKEHCLMGIK GTVRRSTDGD FIHANVDIDL IISEEEEFGS FEKPIEIFHI IEHFCLGRRR LHLFGRDSSI RPGWLTVGPE LTNSNFNSEL YQTYFAEAPA TGCTSRIELL RPKSPPPNSK VLRGRGRGFP RGRGRPR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Non-catalytic component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of mRNAs, a modification that plays a role in the efficiency of mRNA splicing and is required for sex determination. In the heterodimer formed with Ime4/Mettl3, Mettl14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. Required for sex determination and dosage compensation via Sxl alternative splicing: m6A methylation acts as a key regulator of Sxl pre-mRNA and promotes female-specific alternative splicing of Sxl, which determines female physiognomy. M6A methylation is also required for neuronal functions.
Subcellular Location
Nucleus.
Protein Families
MT-A70-like family
Database Links

KEGG: dme:Dmel_CG7818

STRING: 7227.FBpp0079219

UniGene: Dm.351

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