Mettl14 Antibody

Code CSB-PA896115XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Mettl14 Polyclonal antibody
Uniprot No.
Target Names
Mettl14
Alternative Names
Mettl14 antibody; KAR4 antibody; CG7818 antibody; N6-adenosine-methyltransferase non-catalytic subunit antibody; Karyogamy protein 4 antibody; Methyltransferase-like protein 14 antibody; dMettl14 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Mettl14 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Non-catalytic component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of mRNAs, a modification that plays a role in the efficiency of mRNA splicing and is required for sex determination. In the heterodimer formed with Ime4/Mettl3, Mettl14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. Required for sex determination and dosage compensation via Sxl alternative splicing: m6A methylation acts as a key regulator of Sxl pre-mRNA and promotes female-specific alternative splicing of Sxl, which determines female physiognomy. M6A methylation is also required for neuronal functions.
Subcellular Location
Nucleus.
Protein Families
MT-A70-like family
Database Links

KEGG: dme:Dmel_CG7818

STRING: 7227.FBpp0079219

UniGene: Dm.351

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