Recombinant Drosophila melanogaster Microtubule-associated protein futsch (futsch), partial

Code CSB-YP893844DLU
MSDS
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Source Yeast
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Code CSB-EP893844DLU
MSDS
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Source E.coli
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Code CSB-EP893844DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893844DLU
MSDS
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Source Baculovirus
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Code CSB-MP893844DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
futsch
Uniprot No.
Alternative Names
futsch; CG34387Microtubule-associated protein futsch; MAP1B homolog) [Cleaved into: Futsch heavy chain; Futsch light chain LC(f)]
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
During embryogenesis, necessary for dendritic and axonal organization and growth at the neuromuscular junction through the regulation of the synaptic microtubule cytoskeleton. Microtubule hairpin loops are found within a small subset of synaptic boutons at the neuromuscular synapse, these loops are stabilized by Futsch. Loop morphology and dynamics suggest that rearrangement of these microtubule-based loops is a critical component of the process of bouton division and for subsequent nerve-terminal growth and branching. Translation is repressed by Fmr1. Together with Ringer, required for neuromuscular junction (NMJ) bouton growth by regulating synaptic microtubules.
Gene References into Functions
  1. our data are in favor of a model by which Futsch locally stabilizes synaptic active zones, by reinforcing their link with the underlying microtubule cytoskeleton. PMID: 24828631
  2. Microtubule-associated protein 1 function is fulfilled by light chain and heavy chain FUTSCH-encoded precursor polypeptide subunits, identified following proteolytic processing at a conserved cleavage site. PMID: 18419581
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton.
Tissue Specificity
Neuronal cells within the PNS and CNS.
Database Links

KEGG: dme:Dmel_CG34387

STRING: 7227.FBpp0300236

UniGene: Dm.6254

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