Recombinant Drosophila melanogaster Netrin-A (NetA), partial

Code CSB-YP634512DLU
MSDS
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Source Yeast
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Code CSB-EP634512DLU
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Source E.coli
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Code CSB-EP634512DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP634512DLU
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Source Baculovirus
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Code CSB-MP634512DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NetA
Uniprot No.
Alternative Names
NetA; CG18657; Netrin-A
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Netrins control guidance of CNS commissural axons at the midline and peripheral motor axons to their target muscles.
Gene References into Functions
  1. mutation of mushroom body defect (mud) dramatically enhances the phenotype of Netrin or frazzled mutants, resulting in many more axons failing to cross the midline, although mutations in mud alone have little effect. PMID: 26893348
  2. Contact-mediated cell adhesion via Fmi acts in addition to the Net/Fra guidance system during axon pathfinding across the midline, underlining the importance of PCP molecules during vertebrates and invertebrates midline development. PMID: 25440577
  3. adult Drosophila lacking both netrin genes, NetA and NetB, have fertility defects in both sexes together with an inability to fly and reduced viability. PMID: 23977313
  4. In peripodial cells Frazzled is endocytosed and undergoes NetA-dependent degradation, which is required for eversion. PMID: 24225841
  5. These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse. PMID: 24741033
  6. NetA and semaphorin-1a can specifically promote growth through induction of key cellular growth regulators. PMID: 20162636
  7. Misexpression of Netrin-A in oenocytes, secretory cells that lie ventral to the cap cell, leads to aberrant cap cell morphogenesis, suggesting that Netrin-A acts as an instructive cue to direct the growth of the cap cell process. PMID: 20530550
  8. NETRIN serves as a chemo-attractant while SLIT functions antagonistically to NETRIN as a chemo-repellent during embryonic salivary gland migration parallel to the CNS in Drosophila. PMID: 15950216
  9. before reaching the segmental boundary, the longitudinal axons' responsiveness to Netrin presented on the commissure is suppressed by Roundabout, through counteracting Gq signaling PMID: 16341212
  10. the midline signalling systems Slit/Robo and Netrin/Frazzled are the main molecular mechanisms that underlie dendritic targeting with respect to the midline. PMID: 19771146
  11. the medio-lateral positioning of motoneuron dendrites in the leg neuropil is controlled by the midline signalling systems Slit-Robo and Netrin-Fra. PMID: 19771147

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
At the midline of developing CNS at the time of commissure formation and in different subsets of neurons, muscles, and epidermal patches.
Database Links

KEGG: dme:Dmel_CG18657

STRING: 7227.FBpp0073759

UniGene: Dm.2915

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