Recombinant Drosophila melanogaster Nitric oxide synthase (Nos), partial

Code CSB-YP635657DLU
MSDS
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Source Yeast
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Code CSB-EP635657DLU
MSDS
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Source E.coli
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Code CSB-EP635657DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP635657DLU
MSDS
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Source Baculovirus
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Code CSB-MP635657DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nos
Uniprot No.
Alternative Names
Nos; CG6713; Nitric oxide synthase; EC 1.14.13.39; dNOS
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Truncated isoforms (isoform 3-isoform 6) are able to form intracellular complexes with the full-length protein and serve as dominant negative inhibitors of the enzyme activity.
Gene References into Functions
  1. Here we demonstrate that the Drosophila relaxin receptor homolog Lgr3, a leucine-rich repeat-containing G-protein-coupled receptor, is required for Dilp8-dependent growth coordination and developmental delay during the regeneration checkpoint; we demonstrate that Lgr3 activity in both the CNS and PG is necessary for NOS activation in the PG following damage PMID: 27558136
  2. Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration PMID: 26081194
  3. Hb9 governs neuronal specification and differentiation and activates expression of Nos and fd59a in the Drosophila CNS PMID: 24512689
  4. Nitric oxide synthase is not essential for Drosophila development. PMID: 20178753
  5. biochemical characterization of Drosophila nitric oxide synthase PMID: 12804606
  6. Oxygenase domain of Drosophila melanogaster Nos: unique kinetic parameters enable a more efficient NO release. PMID: 17900148
  7. Reductase domain of Drosophila melanogaster Nos: redox transformations, regulation, and similarity to mamalian homologues. PMID: 17900149
  8. The suppressed enzyme expression was shown to affect the cell polarity and movement as well as the structural pattern of organs during Drosophila development. PMID: 19326842

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Protein Families
NOS family
Database Links

KEGG: dme:Dmel_CG6713

STRING: 7227.FBpp0099922

UniGene: Dm.2707

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