Recombinant Drosophila melanogaster Odorant receptor 22a (Or22a), partial

Code CSB-YP305699DLU1
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Source Yeast
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Code CSB-EP305699DLU1
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Source E.coli
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Code CSB-EP305699DLU1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP305699DLU1
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Source Baculovirus
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Code CSB-MP305699DLU1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Or22a
Uniprot No.
Alternative Names
Or22a; AN11; DOR22A.1; dor53; Or22A.1; CG12193; Odorant receptor 22a
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. Involved in the behavioral responses ethyl butyrate and to esters in more general. Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide.
Gene References into Functions
  1. The or22a neuron major projected into glomerulus of DM2 and less projected to VC1 and VC31. PMID: 12555270
  2. odorant receptors thus form ligand-gated channels as well as complexes of odorant-sensing units and cyclic-nucleotide-activated non-selective cation channels PMID: 18408711
  3. signals by opening cation channels, through a mechanism that is largely independent of G proteins PMID: 18625400
  4. Data show that Or22a is expressed in approxiamately 25 olfactory sensory neurons and marks a small glomerulus, DM2. PMID: 18683641
  5. Intraspecific study of 4 natural populations of D melanogaster survey showed a copy-number polymorphism in some of the populations, with the 2 genes, Or22a & Or22b in the long variant and a single chimeric gene, Or22ab present in the short variant. PMID: 18922763

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Insect chemoreceptor superfamily, Heteromeric odorant receptor channel (TC 1.A.69) family, Or2a subfamily
Tissue Specificity
Expressed with Orco in 17-20 sensory neurons on the medial-proximal edge of the antenna. Expressed in the ab3A neuron which responds to ethyl butyrate.
Database Links
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